Novel carbon film induces precocious calcium oscillation to promote neuronal cell maturationopen access
- Authors
- Ludwig, A.; Kesaf, S.; Heikkinen, J.J.; Sukhanova, T.; Khakipoor, S.; Molinari, F.; Pellegrino, C.; Kim, S.I.; Han, J.G.; Huttunen, H.J.; Lauri, S.E.; Franssila, S.; Jokinen, V.; Rivera, C.
- Issue Date
- Oct-2020
- Publisher
- Nature Research
- Citation
- Scientific Reports, v.10, no.1
- Indexed
- SCIE
SCOPUS
- Journal Title
- Scientific Reports
- Volume
- 10
- Number
- 1
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/6498
- DOI
- 10.1038/s41598-020-74535-6
- ISSN
- 2045-2322
- Abstract
- Different types of carbon materials are biocompatible with neural cells and can promote maturation. The mechanism of this effect is not clear. Here we have tested the capacity of a carbon material composed of amorphous sp3 carbon backbone, embedded with a percolating network of sp2 carbon domains to sustain neuronal cultures. We found that cortical neurons survive and develop faster on this novel carbon material. After 3 days in culture, there is a precocious increase in the frequency of neuronal activity and in the expression of maturation marker KCC2 on carbon films as compared to a commonly used glass surface. Accelerated development is accompanied by a dramatic increase in neuronal dendrite arborization. The mechanism for the precocious maturation involves the activation of intracellular calcium oscillations by the carbon material already after 1 day in culture. Carbon-induced oscillations are independent of network activity and reflect intrinsic spontaneous activation of developing neurons. Thus, these results reveal a novel mechanism for carbon material-induced neuronal survival and maturation. © 2020, The Author(s).
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Collections - Graduate School > Advanced Materials Science and Engineering > 1. Journal Articles
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