Polycation-mediated enhancement of retroviral transduction efficiency depends on target cell types and pseudotyped Env proteins: Implication for gene transfer into neural stem cells
- Authors
- Jang, J[Jang, Jiwon]; Lee, J[Lee, Junsup]; Kim, ST[Kim, Seong-Tae]; Lee, KY[Lee, Ki-Young]; Cho, JY[Cho, Jae Youl]; Kweon, DH[Kweon, Dae-Hyuk]; Kwon, ST[Kwon, Suk-Tae]; Koh, YH[Koh, Young Ho]; Kim, S[Kim, Sunyoung]; Yoon, K[Yoon, Keejung]
- Issue Date
- Jun-2012
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Polycation; Polybrene; Retroviral transduction; Ne
- Citation
- NEUROCHEMISTRY INTERNATIONAL, v.60, no.8, pp.846 - 851
- Indexed
- SCIE
SCOPUS
- Journal Title
- NEUROCHEMISTRY INTERNATIONAL
- Volume
- 60
- Number
- 8
- Start Page
- 846
- End Page
- 851
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/65239
- DOI
- 10.1016/j.neuint.2012.02.033
- ISSN
- 0197-0186
- Abstract
- Polycations such as polybrene (PB) are routinely used for most retroviral vector-mediated gene transfer studies because they can increase the infectivity of retroviruses. However, it was not systematically determined if addition of the polycation is an essential prerequisite for all retroviral transductions. To test this, we measured the effects of the polycation on transduction efficiency using various combinations of target cells and pseudotyped viral envelope (Env) proteins. Here, we show polycations do not always increase retroviral transduction efficiency and that their enhancing effect depends on both the type of target cells and Env proteins. The findings presented here also suggest that high transduction rates can be achieved in primary neural stem cells in vitro and in vivo by choosing an appropriate Env protein for pseudotyping without using polycations which are potentially toxic to primary cells and may change the intrinsic characteristics of cells. (C) 2012 Elsevier Ltd. All rights reserved.
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- Appears in
Collections - Biotechnology and Bioengineering > Department of Genetic Engineering > 1. Journal Articles
- Biotechnology and Bioengineering > Integrative Biotechnology > 1. Journal Articles
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