Anti-inflammatory activity of ethanol extract derived from Phaseolus angularis beans
- Authors
- Yu, T[Yu, Tao]; Ahn, HM[Ahn, Hyo Min]; Shen, T[Shen, Ting]; Yoon, K[Yoon, Keejung]; Jang, HJ[Jang, Hyun-Jae]; Lee, YJ[Lee, Yong Jin]; Yang, HM[Yang, Hyun Mo]; Kim, JH[Kim, Jae Hun]; Kim, C[Kim, Changhyuk]; Han, MH[Han, Moon Hi]; Cha, SH[Cha, Sang-hun]; Kim, TW[Kim, Tae Wong]; Kim, SY[Kim, Sun Young]; Lee, J[Lee, Jaehwi]; Cho, JY[Cho, Jae Youl]
- Issue Date
- 11-Oct-2011
- Keywords
- CREB; Inflammatory Mediators; Macrophages; NF-B; p38; Phaseolus angularis; Syk
- Citation
- JOURNAL OF ETHNOPHARMACOLOGY, v.137, no.3, pp.1197 - 1206
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF ETHNOPHARMACOLOGY
- Volume
- 137
- Number
- 3
- Start Page
- 1197
- End Page
- 1206
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/68679
- DOI
- 10.1016/j.jep.2011.07.048
- ISSN
- 0378-8741
- Abstract
- Ethnopharmacological significance: Phaseolus angularis Wight (adzuki bean) is an ethnopharmacologically well-known folk medicine that is prescribed for infection, edema, and inflammation of the joints, appendix, kidney and bladder in Korea, China and Japan. Aim of study: The anti-inflammatory effect of this plant and its associated molecular mechanisms will be investigated. Materials and methods: The immunomodulatory activity of Phaseolus angularis ethanol extract (Pa-EE) in toll like receptor (TLR)-activated macrophages induced by ligands such as lipopolysaccharide (LPS), Poly (I:C), and pam3CSK was investigated by assessing nitric oxide (NO) and prostaglandin (PG)E(2) levels. To identify which transcription factors such as nuclear factor (NF)-kappa B and their signaling enzymes can be targeted to Pa-EE, biochemical approaches including reporter gene assays, immunoprecipitation, kinase assays, and immunoblot analyses were also employed. Finally, whether Pa-EE was orally available, ethanol (EtOH)/hydrochloric acid (HCl)-induced gastritis model in mice was used. Results: Pa-EE dose-dependently suppressed the release of PGE(2) and NO in LPS-, Poly(I:C)-, and pam3CSK-activated macrophages. Pa-EE strongly down-regulated LPS-induced mRNA expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2. Interestingly, Pa-EE markedly inhibited NF-kappa B, activator protein (AP)-1, and cAMP response element binding protein (CREB) activation; further, according to direct kinase assays and immunoblot analyses. Pa-EE blocked the activation of the upstream signaling molecules spleen tyrosine kinase (Syk), p38, and transforming growth factor beta-activated kinase 1 (TAK1). Finally, orally administered Pa-EE clearly ameliorated EtOH/HCl-induced gastritis in mice. Conclusion: Our results suggest that Pa-EE can be further developed as a promising anti-inflammatory remedy because it targets multiple inflammatory signaling enzymes and transcription factors. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Biotechnology and Bioengineering > Integrative Biotechnology > 1. Journal Articles
- Biotechnology and Bioengineering > Department of Genetic Engineering > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.