Mycobacterial Infection after Intravesical Bacillus Calmette-Guerin Treatment for Bladder Cancer: A Case Report
- Authors
- Park, CH[Park, Chang-Hun]; Fang, MA[Fang, Mi Ae]; Ahn, YH[Ahn, Yoon Hee]; Hwang, YY[Hwang, Yu-Yean]; Ki, CS[Ki, Chang-Seok]; Lee, NY[Lee, Nam Yong]
- Issue Date
- Jul-2011
- Keywords
- BCG; Bladder cancer; Multiplex-PCR; Mycobacterium bovis; Sequence analysis
- Citation
- KOREAN JOURNAL OF LABORATORY MEDICINE, v.31, no.3, pp.197 - 200
- Indexed
- SCIE
SCOPUS
- Journal Title
- KOREAN JOURNAL OF LABORATORY MEDICINE
- Volume
- 31
- Number
- 3
- Start Page
- 197
- End Page
- 200
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/69534
- DOI
- 10.3343/kjlm.2011.31.3.197
- ISSN
- 1598-6535
- Abstract
- Bacillus Calmette-Guerin (BCG) has been traditionally used as a vaccine against tuberculosis. Further, intravesical administration of BCG has been shown to be effective in treating bladder cancer. Although BCG contains a live attenuated strain of Mycobacterium bovis, complications such as M. bovis BCG infection caused by BCG administration are extremely rare. Here, we report a case of BCG infection occurring after intravesical BCG therapy. A 67-yr-old man presented with azotemia and weight loss. He had been diagnosed with bladder cancer 4 yr back, and had undergone transurethral resection of the bladder tumor and intravesical BCG (Tice strain) therapy at that time. An acid-fast bacterial strain was isolated from his urine sample. We did not detect Mycobacterium tuberculosis protein 64 (MPT-64) antigen in the isolates obtained from his sample, and multiplex PCR and PCR-reverse blot hybridization assay indicated that the isolate was a member of the M. tuberculosis complex, but was not M:tuberculosis. Finally, sequence analysis of 165 ribosomal RNA and DNA gyrase, subunit B (gyrB) suggested that the organism was M. bovis or M. bovis BCG. Although we could not confirm that M. bovis BCG was the causative agent, the results of the 3 molecular methods and the MPT-64 antigen assay suggest this finding. This is an important finding, especially because M. bovis BCG cannot be identified using common commercial molecular genetics tools.
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Collections - Medicine > Department of Medicine > 1. Journal Articles
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