Treadmill exercise suppresses muscle cell apoptosis by increasing nerve growth factor levels and stimulating p-phosphatidylinositol 3-kinase activation in the soleus of diabetic rats
- Authors
- Chae, CH[Chae, Chang-Hun]; Jung, SL[Jung, Sung-Lim]; An, SH[An, Sang-Hyun]; Jung, CK[Jung, Chan-Kyoung]; Nam, SN[Nam, Sang-Nam]; Kim, HT[Kim, Hyun-Tae]
- Issue Date
- Jun-2011
- Keywords
- Apoptotic cell death; Diabetic rats; Nerve growth factor; p-PI3-K; Treadmill exercise
- Citation
- JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY, v.67, no.2, pp.235 - 241
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
- Volume
- 67
- Number
- 2
- Start Page
- 235
- End Page
- 241
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/69722
- DOI
- 10.1007/s13105-010-0068-9
- ISSN
- 1138-7548
- Abstract
- We investigated the effects of treadmill exercise performed regularly for 6 weeks on the levels of nerve growth factor (NGF), tyrosine kinase A and p75 receptors, phosphatidylinositol 3-kinase (PI3-K), mitogen-activated protein kinase/extracellular signal-regulated kinase (Erk) 1,2, cyclic AMP response element-binding protein (CREB), and caspase-3 in the soleus of rats with streptozotocin (STZ)-induced diabetes. Thirty-two male Sprague-Dawley rats were divided into the following four groups: (1) normal control group (NCG; n = 8), (2) normal exercise group (NEG; n = 8), (3) diabetes control group (DCG; n = 8), and (4) diabetes exercise group (DEG; n = 8). Diabetes was induced by intraperitoneal injection of STZ (55 mg/kg dissolved in 0.05 M citrate buffer, pH 4.5). Rats were subjected to treadmill exercise 5 days a week for 6 weeks. The protein level of NGF significantly increased in the NEG and DEG (p < 0.001), whereas the levels of tyrosine kinase A and p75 receptors significantly increased in the NEG (p < 0.001). The levels of t-PI3-K, p-PI3-K, and p-CREB, and the p-CREB/t-CREB ratio significantly increased in the NEG (p < 0.001, respectively). The p-PI3-K/t-PI3-K ratio significantly increased in the DEG (p < 0.001). The p-Erk1/t-Erk1 ratio significantly increased in the NEG (p < 0.001), whereas the p-Erk2/t-Erk2 ratio significantly decreased in the DCG and DEG (p < 0.001). The caspase-3 level significantly increased in the DCG compared with that in the DEG (p < 0.001). These results suggest that treadmill exercise increases NGF levels and accelerates p-PI3-K activation in order to suppress apoptotic cell death in the soleus muscle of diabetic rats.
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Collections - Sport Science > Department of Sport Science > 1. Journal Articles
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