Molecular Detection of Tyrosine Hydroxylase in the Peripheral Blood of Patients with Neuroblastoma: Useful at Diagnosis but not Predictive of Subsequent Relapse During Off-Therapy Follow-Up
- Authors
- Lee, ST[Lee, Seung-Tae]; Ki, CS[Ki, Chang-Seok]; Sung, KW[Sung, Ki Woong]; Kim, HJ[Kim, Hee-Jin]; Kim, JW[Kim, Jong-Won]; Kim, SH[Kim, Sun-Hee]; Lee, SH[Lee, Soo Hyun]; Yoo, KH[Yoo, Keon Hee]; Koo, HH[Koo, Hong Hoe]; Kim, JY[Kim, Ju Youn]; Cho, EJ[Cho, Eun Joo]
- Issue Date
- Feb-2011
- Keywords
- minimal residual disease; neuroblastoma; peripheral blood; RT-PCR; tyrosine hydroxylase
- Citation
- PEDIATRIC HEMATOLOGY AND ONCOLOGY, v.28, no.1, pp.16 - 23
- Indexed
- SCIE
SCOPUS
- Journal Title
- PEDIATRIC HEMATOLOGY AND ONCOLOGY
- Volume
- 28
- Number
- 1
- Start Page
- 16
- End Page
- 23
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/70943
- DOI
- 10.3109/08880018.2010.514694
- ISSN
- 0888-0018
- Abstract
- In the present study, the authors analyzed the tyrosine hydroxylase (TH) expression in peripheral blood (PB) of neuroblastoma (NB) patients and investigated the clinical implications. From April 2005 to October 2008, a total of 683 PB specimens (64 at diagnosis, 244 during chemotherapy, 355 during off-therapy follow-up, and 20 at relapse) acquired from 141 patients were investigated. TH expression was measured by quantitative reverse transcriptase-polymerase chain reaction (RTPCR). TH-positive rate at diagnosis (21.4%) was higher than those during chemotherapy (0.8%) or off-therapy follow-up (1.7%). TH expression at diagnosis was associated with high-risk features (ie, advanced stage, older age, unfavorable pathology, and amplified N-myc) and the probability of 3-year relapse-free survival in the TH-positive patients was lower than in the TH-negative patients (45.8% +/- 27.8% versus 95.8% +/- 5.7%, P < .001). TH expression was positive in only 6 specimens during off-therapy follow-up. However, tumor relapse occurred in only 2 out of 6 TH-positive patients. In addition, TH expression was negative during previous off-therapy follow-up, prior to relapse, in 8 out of 10 relapsed patients. Whereas TH expression in PB at diagnosis was associated with high-risk features and a poorer outcome, TH expression during off-therapy follow-up had very limited value for the prediction of a subsequent relapse.
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Collections - Medicine > Department of Medicine > 1. Journal Articles
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