Isolation of RNA Aptamers Targeting HER-2-overexpressing Breast Cancer Cells Using Cell-SELEX
- Authors
- Kang, HS[Kang, Hye Suk]; Huh, YM[Huh, Yong Min]; Kim, S[Kim, Soyoun]; Lee, D[Lee, Dong-ki]
- Issue Date
- Aug-2009
- Publisher
- KOREAN CHEMICAL SOC
- Keywords
- HER-2; Cell-SELEX; Aptamer; Breast cancer; siRNA
- Citation
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.30, no.8, pp.1827 - 1831
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY
- Volume
- 30
- Number
- 8
- Start Page
- 1827
- End Page
- 1831
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/77360
- ISSN
- 0253-2964
- Abstract
- Ligand molecules that can recognize and interact with cancer cell surface marker proteins with high affinity and specificity should greatly aid the development of novel cancer diagnostics and therapeutics. HER-2/ErbB2/Neu (HER-2), a member of the epidermal growth factor receptor family, is specifically overexpressed on the surface of breast cancer cells and serves as both a useful biomarker and a therapeutic target for breast cancer. In this study, we aimed to isolate RNA aptamers that specifically bind to a HER-2-overexpressing human breast cancer cell line, SK-BR-3, using Cell-SELEX strategy. The selected aptamers showed strong affinity to SK-BR-3, but not to MDA-MB-231, a HER-2-underexpressing breast cancer cell line. In addition, we confirmed the specific targeting of HER-2 receptor by aptamers using an unrelated mouse cell line overexpressing human HER-2 receptor. The HER-2-targeting RNA aptamers could become a useful reagent for the development of breast cancer diagnostics and therapeutics.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Science > Department of Chemistry > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/77360)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.