Synthesis and base pairing properties of DNA-RNA heteroduplex containing 5-hydroxyuridine
- Authors
- Cui, S[Cui, Song]; Kim, YH[Kim, Yong-Hoon]; Jin, CH[Jin, Cheng-Hao]; Kim, SK[Kim, Sang Kook]; Rhee, MH[Rhee, Man-hee]; Kwon, OS[Kwon, Oh-Shin]; Moon, BJ[Moon, Byung Jo]
- Issue Date
- 30-Jun-2009
- Publisher
- KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
- Keywords
- Base pairing; CD spectra; Melting temperature; Oligoribonucleotide; 5-Hydroxyuridine
- Citation
- BMB REPORTS, v.42, no.6, pp.373 - 379
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BMB REPORTS
- Volume
- 42
- Number
- 6
- Start Page
- 373
- End Page
- 379
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/77579
- ISSN
- 1976-6696
- Abstract
- 5-Hydroxyuridine (5-OHU) is a major lesion of uridine and cytosine produced in RNA by various chemical oxidants. To elucidate its biochemical and biophysical effects on RNA replication, the site-specifically modified oligoribonucleotides containing 5-OHU were synthesized with C5-hydroxy-5'-O-DMTr-2'-TBDMS-uridine phosphoramidite using automated solid phase synthesis. The base-pairing properties of nucleotides opposite 5-OHU in 24 mer oligoribonulcleotides with dNTP were studied using three reverse transcriptases (Super-Script (TM) II-, AMV-, MMLV-RT) in cDNA synthesis. Adenine as well as guanine was incorporated preferentially by all reverse transcriptases. In the UV-melting temperature experiment, the results from the relative stabilities of the base pairs were A : 5-OHU > G : 5-OHU > T : 5-OHU approximate to C : 5-OHU. Circular Dichroism (CD) studies showed that DNA-RNA containing 5-OHU heteroduplexes exhibit a similar conformation between the A-type RNA and B-type DNA. These results suggest that 5-OHU from oxidative damage was mainly influenced by adenine mismatch. [BMB reports 2009; 42(6): 373-379]
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Collections - Pharmacy > Department of Pharmacy > 1. Journal Articles
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