Annexin I Stimulates Insulin Secretion through Regulation of Cytoskeleton and PKC Activity
- Authors
- Kang, NN[Kang, Na-na]; Won, JH[Won, Jong Hak]; Park, YM[Park, Young Min]
- Issue Date
- 31-Mar-2009
- Publisher
- ZOOLOGICAL SOC KOREA
- Keywords
- Anx I; insulin secretion; cytoskeleton; perifusion; reserve pool
- Citation
- ANIMAL CELLS AND SYSTEMS, v.13, no.1, pp.17 - 23
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- ANIMAL CELLS AND SYSTEMS
- Volume
- 13
- Number
- 1
- Start Page
- 17
- End Page
- 23
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/78227
- DOI
- 10.1080/19768354.2009.9647190
- ISSN
- 1976-8354
- Abstract
- In previous studies, we found that Annexin I (Anx I) was co-secreted with insulin in response to glucose, and that extracellular Anx I stimulated the release of insulin via the Anx I binding site in rat pancreatic islets and the P-cell line. However, the role that Anx I plays in the insulin secretion was not established. Therefore, in this study, we evaluated the insulin secretion pattern in response to Anx I and the involvement of the cytoskeleton or PKC in Anx I-stimulated insulin secretion in MIN6N8a cells. The peak time of insulin secretion in response to Anx I treatment corresponded with the second phase insulin secretion by glucose in the perifused pseudoislets. In addition, Anx I-stimulated insulin secretion was not affect by readily releasable pool depletion. Taken together, these findings indicate that Anx I treatment was associated with movement of the reserve pool of insulin. Furthermore, Anx I-stimulated insulin secretion was attenuated by treatment with a microfilament inhibitor, cytochalasin B, as well as by PKC down regulation. These results indicate that Anx I may be a regulator of second phase insulin secretion.
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Collections - Science > Department of Biological Science > 1. Journal Articles
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