HPLC analysis of caffeoylquinic acids in the extract of Cacalia firma and peroxynitrite scavenging rffect

Abstract

Six caffeoylquinic acids of Cacalia firma (Komarov) Nakai (Compositae) leaves were identified using standard compounds by HPLC. Each content of those compounds in dried weight was determined as follows: 3,4-di-O-caffeoylquinic acid (1.44±0.04 mg/g of dried weight), 3,5-di-O-caffeoyl-muce-quinic acid (2.47±0.12 mg/g), 3,5-di-O-caffeoylquinic acid (3.74±0.24 mg/g), 5-caffeoylquinic acid (chlorogenic acid, 5.20±0.09 mg/g), 3-caffeoylquinic acid (1.35±0.01 mg/g) and 3-O-p-coumaroylquinic acid (3.84±0.25 mg/g). The total content of six caffeoylquinic acids in the plant material was calculated as 18.05±0.69 mg/g while the percentage of the six compounds in the MeOH extract was calculated as 30.85±1.18%. The IC50 value of the MeOH extract scavenging peroxynitrite (ONOO-) was shown as 3.22±0.57 μg/ml.