Isolation of RNA aptamers targeting HER-2-overexpressing breast cancer cells using cell-SELEX
- Authors
- Kang, H.S.[Kang, H.S.]; Huh, Y.M.[ Huh, Y.M.]; Kim, S.[ Kim, S.]; Lee, D.-k.[Lee, D.-k.]
- Issue Date
- 2009
- Keywords
- Aptamer; Breast cancer; Cell-SELEX; HER-2; siRNA
- Citation
- Bulletin of the Korean Chemical Society, v.30, no.8, pp.1827 - 1831
- Indexed
- SCOPUS
KCI
- Journal Title
- Bulletin of the Korean Chemical Society
- Volume
- 30
- Number
- 8
- Start Page
- 1827
- End Page
- 1831
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/79625
- DOI
- 10.5012/bkcs.2009.30.8.1827
- ISSN
- 0253-2964
- Abstract
- Ligand molecules that can recognize and interact with cancer cell surface marker proteins with high affinity and specificity should greatly aid the development of novel cancer diagnostics and therapeutics. HER-2/ErbB2/Neu (HER-2), a member of the epidermal growth factor receptor family, is specifically overexpressed on the surface of breast cancer cells and serves as both a useful biomarker and a therapeutic target for breast cancer. In this study, we aimed to isolate RNA aptamers that specifically bind to a HER-2-overexpressing human breast cancer cell line, SK-BR-3, using Cell-SELEX strategy. The selected aptamers showed strong affinity to SK-BR-3, but not to MDA-MB-231, a HER-2-underexpressing breast cancer cell line. In addition, we confirmed the specific targeting of HER-2 receptor by aptamers using an unrelated mouse cell line overexpressing human HER-2 receptor. The HER-2-targeting RNA aptamers could become a useful reagent for the development of breast cancer diagnostics and therapeutics.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Science > Department of Chemistry > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.