Synthesis and base pairing properties of DNA-RNA heteroduplex containing 5-hydroxyuridine
- Authors
- Cui, S.[Cui, S.]; Kim, Y.-H.[ Kim, Y.-H.]; Jin, C.-H.[Jin, C.-H.]; Kim, S.K.[ Kim, S.K.]; Rhee, M.-H.[ Rhee, M.-H.]; Kwon, O.-S.[ Kwon, O.-S.]; Moon, B.J.[ Moon, B.J.]
- Issue Date
- 2009
- Keywords
- 5-Hydroxyuridine; Base pairing; CD spectra; Melting temperature; Oligoribonucleotide
- Citation
- BMB Reports, v.42, no.6, pp.373 - 379
- Indexed
- SCOPUS
KCI
- Journal Title
- BMB Reports
- Volume
- 42
- Number
- 6
- Start Page
- 373
- End Page
- 379
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/79636
- ISSN
- 1976-6696
- Abstract
- 5-Hydroxyuridine (5-OHU) is a major lesion of uridine and cytosine produced in RNA by various chemical oxidants. To elucidate its biochemical and biophysical effects on RNA replication, the site-specifically modified oligoribonucleotides containing 5-OHU were synthesized with C5-hydroxy-5'-ODMTr-2'-TBDMS-uridine phosphoramidite using automated solid phase synthesis. The base-pairing properties of nucleotides opposite 5-OHU in 24 mer oligoribonulcleotides with dNTP were studied using three reverse transcriptases (Super-ScriptTMII-, AMV-, MMLV-RT) in cDNA synthesis. Adenine as well as guanine was incorporated preferentially by all reverse transcriptases. In the UV-melting temperature experiment, the results from the relative stabilities of the base pairs were A: 5-OHU > G: 5-OHU > T: 5-OHU ≈ C: 5-OHU. Circular Dichroism (CD) studies showed that DNA-RNA containing 5-OHU heteroduplexes exhibit a similar conformation between the A-type RNA and B-type DNA. These results suggest that 5-OHU from oxidative damage was mainly influenced by adenine mismatch.
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- Appears in
Collections - Pharmacy > Department of Pharmacy > 1. Journal Articles
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