The omentum culture using cell-PLGA sheet

Abstract

The cell and porous scaffold are main components of tissue engineering. The cell seeded-porous scaffold is cultured in vitro to make tissue-like structure and then transplanted into in vivo to reconstruct the injured tissue and organ. However. in vitro culture method can not supply sufficient blood vessels and nutrients and growth factors for tissue formation and also require long cell culture time. For these reasons, in vivo culture method using omentum has been studied, because the omentum can supply many blood vessels and various nutrient and oxygen. But once the porous scaffold transplanted into in vivo, the intrusion of other kinds of cells into scaffold pores and the excessive accumulation of extracellular matrix are inevitable. As a result merits of the use of omentum are not worth. To avoid these problems and use omentum effectively, stable cell layer composed with cells have to be made. In this experiment, we made stable cell layer using PLGA sheet and used Q dot for identification of in vivo cell distribution.