A novel lactocin-based solid lipid nanoparticles for smart probiotic nanofood : Rheological, mucoadhesive and in vitro release properties

Abstract

The aim of the present work was to prepare and evaluate oral mucoadhesive sustained release nanoparticles of lactocin(CBT-LP2) these nanoparticles Could then be used to improve patient compliance by simplifying the administration of lactocin, improving the therapeutic effect and reducing the dose related side effects. CBT-LP2 containing solid lipid nanoparticles(SLN) were prepared by ultrasonication using soybean lecithin as a stabilizing agent. The results showed that this method was reproducible, easily performed and led to efficient entrapment of CBT-LP2 as well as formation of spherical particles ranging from 80-200 nm. In addition, process variables, including the effect of gliadin concentration and the effect of surfactant. were also evaluated with respect to the percent CBT-LP2 entrapment and percent yields. The maximum percent CBT-LP2 entrapment and percent yield were approximately 73 and 88%, respectively. The Sustained release behaviors of the gliadin nanoparticles and the smart probiotic nanofood(SPN) were evaluated in both phosphate buffered saline(pH 7.4) and simulated gastric fluid(pH 1.2) at 37 +/- 1 degrees C. Their mucoadhesive properties were determined by in vitro and in vivo methods. The shelf life of prepared SPN was determined by storage at various temperatures in simulated gastric fluid(pH 1.2) with and without added enzymes.