Target metabolic profiling analysis of free amino acids in plasma as EOC/TBDMS derivatives by GC-SIM-MS
- Authors
- Paik, MJ[Paik, Man-Jeong]; Moon, SM[Moon, Seung-Min]; Kim, KR[Kim, Kyoung-Rae]; Choi, S[Choi, Sangdun]; Ahn, YH[Ahn, Young-Hwan]; Lee, G[Lee, Gwang]
- Issue Date
- Apr-2008
- Publisher
- JOHN WILEY & SONS LTD
- Keywords
- target metabolic profiling analysis; gas chromatography-mass spectrometry in selected ion monitoring mode; ethoxycarbonyl/tert-butyldimethylsilyl derivatives; free amino acids; plasma
- Citation
- BIOMEDICAL CHROMATOGRAPHY, v.22, no.4, pp.339 - 342
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOMEDICAL CHROMATOGRAPHY
- Volume
- 22
- Number
- 4
- Start Page
- 339
- End Page
- 342
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/81766
- DOI
- 10.1002/bmc.939
- ISSN
- 0269-3879
- Abstract
- Metabolic profiling analysis of free amino acids (AAs) in plasma (20 mu L) was performed by gas chromatography-mass spectrometry in selected ion monitoring mode after ethoxycarbonyl/tert-butyldimethylsilyl derivatives. Characteristic fragment ions, including [M - 57](+) ions, permitted sensitive and selective detection of most of the AAs in the presence of co-extracted carboxylic acids, including free fatty acids, at much higher levels. The overall method was linear (r >= 0.9991), reproducible (relative standard deviation = 2.3-8.8%) and accurate (relative error = -7.3-7.7%) with detection limits of 0.01-1.9 ng/mL. A total of 18 AAs, 15 protein AAs and three nonprotein AAs were quantitatively screened in a normal human plasma sample. This selective and simple method using a minimal sample volume was effective for the quantitation of plasma free AAs. Copyright (c) 2007 John Wiley & Sons, Ltd.
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