Whitening effect of adipose-derived stem cells: A critical role of TGF-beta 1
- Authors
- Kim, WS[Kim, Won-Serk]; Park, SH[Park, So-Hyun]; Ahn, SJ[Ahn, Se-Jin]; Kim, HK[Kim, Hyung-Ki]; Park, JS[Park, Jeong-Soo]; Lee, GY[Lee, Ga-Young]; Kim, KJ[Kim, Kea-Jeung]; Whang, KK[Whang, Kyu-Kwang]; Kang, SH[Kang, Seung-Hee]; Park, BS[Park, Byung-Soon]; Sung, JH[Sung, Jong-Hyuk]
- Issue Date
- Apr-2008
- Publisher
- PHARMACEUTICAL SOC JAPAN
- Keywords
- adipose-derived stem cell; melanin; tyrosinase; tyrosinase-related protein 1 (TRP1); transforming growth factor-beta 1
- Citation
- BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.31, no.4, pp.606 - 610
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOLOGICAL & PHARMACEUTICAL BULLETIN
- Volume
- 31
- Number
- 4
- Start Page
- 606
- End Page
- 610
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/81767
- DOI
- 10.1248/bpb.31.606
- ISSN
- 0918-6158
- Abstract
- It has been demonstrated that adipose-derived stem cells (ADSCs) secrete cytokines and exhibit diverse pharmacological actions. The present study examined the unknown pharmacological action of ADSCs regarding whitening effects. A conditioned medium of ADSCs (ADSC-CM) was harvested and the whitening effect of ADSC-CM was studied in melanoma B16 cells. ADSC-CM treatment inhibited the synthesis of melanin and the activity of tyrosinase in a dose dependent manner. To clarify the underlying mechanisms of the whitening action of ADSCs, protein levels of melanogenic proteins were measured by Western blot. Although expressions of microphthalmia-associated transcription factor and tyrosinase-related protein 2 (TRP2) remained unchanged, those of tyrosinase and TRP1 were down-regulated. Transforming growth factor-beta 1 (TGF-beta 1), a potent regulator of melanogenic proteins, was neutralized by the addition of a blocking antibody to ADSC-CM, and down-regulated expression of tyrosinase and TRP1 was almost reversed. Collectively, these results indicate that secretary factors of ADSC inhibit melanin synthesis by down-regulating the expression of tyrosinase and TRP1, which are mainly mediated by TGF-beta 1.
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Collections - Medicine > Department of Medicine > 1. Journal Articles
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