Effects of cardiotrophin-1 on adriamycin-induced apoptosis in H9c2 cardiomyoblasts
- Authors
- Shin, J.-O.[Shin, J.-O.]; Ju, E.-S.[ Ju, E.-S.]; Song, H.-M.[ Song, H.-M.]; Yun, S.-H.[ Yun, S.-H.]; Lim, B.-K.[ Lim, B.-K.]; Choi, J.-H.[Choi, J.-H.]; Kim, D.-K.[Kim, D.-K.]; Jeon, E.-S.[Jeon, E.-S.]
- Issue Date
- 2008
- Keywords
- Adriamycin; Apoptosis; Cardiotrophin-1; Cell protection
- Citation
- Korean Circulation Journal, v.38, no.5, pp.264 - 269
- Indexed
- SCOPUS
KCI
- Journal Title
- Korean Circulation Journal
- Volume
- 38
- Number
- 5
- Start Page
- 264
- End Page
- 269
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/83052
- DOI
- 10.4070/kcj.2008.38.5.264
- ISSN
- 1738-5520
- Abstract
- Background and Objectives: Adriamycin (doxorubicin, ADR) is a highly effective anti-neoplastic drug, but its clinical use is limited by its adverse side effects on the heart. Cardiotrophin (CT-1), a potent cardiac survival factor, is capable of inhibiting apoptosis in cardiac myocytes. The aim of this study was to investigate the cytoprotective effects of CT-1 against ADR-induced apoptosis in vitro. Materials and Methods: We determined a reasonable ADR concentration for inducing cell death by utilizing a cell survival test performed in a dose-dependent manner. To determine the requirements for apoptosis in ADR-treated cardiac myocytes (H9c2 cells), we examined the effect of CT-1 on survival and apoptotic changes using a cell counting kit (CCK), RT-PCR, and Western blotting. Results: In analyzing cell survival as determined by CCK, ADR-induced cell death was found to occur in a dose-dependent manner (50% death at 24 hours after 2 μM of ADR), and ADR was shown to decrease procaspase-3. On RT-PCR, expression of Bax-α mRNA increased and Bcl-2 decreased during the 24 hours after ADR treatment. Consequently, the ratio of Bax-α/Bcl-2 mRNA peaked at 24 hours after ADR treatment. In contrast, CT-1 effectively attenuated the ADR-induced cell death in a dose-dependent manner. The changes in Bax-α and Bcl-2 mRNA expression after ADR treatment were reversed by CT-1 (1 ng/mL) treatment. The protein levels of procaspase-3 decreased after ADR treatment, an effect which was reversed by CT-1 treatment. Akt phosphorylation was also increased by CT-1, demonstrating that CT-1 inhibited apoptosis induced by ADR. Conclusion: These data demonstrated that ADR-induced apoptosis of cardiomyocytes can be prevented by CT-1; therefore, it may be possible to use CT-1 as a cardioprotective agent during ADR chemotherapy in patients with cancer. Copyright © 2008 The Korean Society of Cardiology.
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Collections - Medicine > Department of Medicine > 1. Journal Articles
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