QCM-D analysis of binding mechanism of phage particles displaying a constrained heptapeptide with specific affinity to SiO2 and TiO2
- Authors
- Chen, HB[Chen, Haibin]; Su, XD[Su, Xiaodi]; Neoh, KG[Neoh, Koon-Gee]; Choe, WS[Choe, Woo-Seok]
- Issue Date
- 15-Jul-2006
- Publisher
- AMER CHEMICAL SOC
- Citation
- ANALYTICAL CHEMISTRY, v.78, no.14, pp.4872 - 4879
- Indexed
- SCIE
SCOPUS
- Journal Title
- ANALYTICAL CHEMISTRY
- Volume
- 78
- Number
- 14
- Start Page
- 4872
- End Page
- 4879
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/87013
- DOI
- 10.1021/ac0603025
- ISSN
- 0003-2700
- Abstract
- A growing number of peptides capable of specifically recognizing inorganic materials have been reported, incrementally increasing the potential to harness peptides as a biological linker to bridge biomolecules and inorganic materials at nanometer scale. In this study, we identified disulfide bond constrained heptapeptides with specific binding affinity to SiO2 and TiO2 using a phage display technique. Interestingly, two of the phage surface displayed peptides enriched with basic amino acid residues, STB1 (HKKPSKS) and STB2 (TKRNNKR), showed a cross binding affinity to both metal oxides. To understand the underlying binding mechanism, binding behaviors of phage particles harboring the STB1 ( a high-frequency heptapeptide exhibiting dual binding affinity to both metal oxides) were investigated in a wide pH range using quartz crystal microbalance with energy dissipation measurement (QCM-D). It was found that the binding of STB1-harboring phages to the two metal oxides was clearly mediated by the peptide moiety displayed on the phage surface in a pH-dependent manner, indicating that the binding is largely governed by electrostatic interaction. Furthermore, the interpretation of QCM-D signals (i.e., frequency shift and dissipation shift), with the aid of AFM image analysis of the phage particles bound on the surface of the two metal oxides, elucidated whether the nature of phage ( or the displayed peptide) binding to the metal oxides is largely specific or nonspecific.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Engineering > School of Chemical Engineering > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.