Development of a Pumpless Microfluidic System to Study the Interaction between Gut Microbes and Intestinal Epithelial Cells
- Authors
- Lee, SY[Lee, Seung Yeon]; Byun, HJ[Byun, Hyun Jae]; Choi, H[Choi, Heelak]; Won, JI[Won, Jong-In]; Han, J[Han, Jeonghun]; Park, S[Park, Sungsu]; Kim, D[Kim, Donghyun]; Sung, JH[Sung, Jong Hwan]
- Issue Date
- Apr-2022
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
- Keywords
- gut microbiota; gut-on-a-chip; oxygen gradient
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.27, no.2, pp.221 - 233
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- Volume
- 27
- Number
- 2
- Start Page
- 221
- End Page
- 233
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/96929
- DOI
- 10.1007/s12257-021-0268-x
- ISSN
- 1226-8372
- Abstract
- Gut microbiota dysbiosis and intestinal epithelial cell barrier dysfunction are associated with diseases such as inflammatory bowel disease and type 2 diabetes. To study the interaction of gut microbes and intestinal epithelial cells, in vitro model systems for co-culturing intestinal cells and gut microbes are essential. Since most gut microorganisms are anaerobic bacteria, it is difficult to co-culture microbes with intestinal epithelial cells that require oxygen during culture. We have developed a novel gut microbialepithelial cell (GMEC) co-culture system, a microfluidic chip in an anaerobic chamber with oxygen gradient within the chip. Appropriate co-culture flow rates were determined based on the experimental observation as well as fluid dynamics and molecular transport simulations. Using GMEC system, Escherichia coli and Lactobacillus plantarum were co-cultured with gut epithelial cell line, Caco-2. We expect that our GMEC co-culture system can potentially be a useful platform for studying the interaction between intestinal epithelial cells and gut microbes.
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Collections - Engineering > School of Mechanical Engineering > 1. Journal Articles
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