Putative Role of a Streptomyces coelicolor-Derived alpha-Mannosidase in Deglycosylation and Antibiotic Production
DC Field | Value | Language |
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dc.contributor.author | Rajesh, Thangamani | - |
dc.contributor.author | Jeon, Jong-Min | - |
dc.contributor.author | Song, Eunjung | - |
dc.contributor.author | Park, Hae-Min | - |
dc.contributor.author | Seo, Hyung Min | - |
dc.contributor.author | Kim, Hyun-Joong | - |
dc.contributor.author | Yi, Da-Hye | - |
dc.contributor.author | Kim, Yong-Hyun | - |
dc.contributor.author | Choi, Kwon-Young | - |
dc.contributor.author | Kim, Yun-Gon | - |
dc.contributor.author | Park, Hyung-Yeon | - |
dc.contributor.author | Lee, Yoo Kyung | - |
dc.contributor.author | Yang, Yung-Hun | - |
dc.date.available | 2018-05-09T11:16:04Z | - |
dc.date.created | 2018-04-17 | - |
dc.date.issued | 2014-02 | - |
dc.identifier.issn | 0273-2289 | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/10132 | - |
dc.description.abstract | SCO0948 was found to be the single open reading frame annotated to encode an alpha-mannosidase (AM1) in Streptomyces coelicolor M145. To characterize the protein, we overexpressed SCO0948 in Escherichia coli BL21(DE3). Recombinant AM1, with a molecular weight of 110 kDa, exhibited alpha-mannosidase activity toward 4-nitrophenyl-alpha-D-mannopyranoside with a K-m of 4.61 mM, a V-max of 101.6 mM/min, and a specific activity of 47.96 U/mg. Treatment of ovalbumin, a glycoprotein, with AM1 resulted in partial deglycosylation, as assessed by glycostaining and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The S. coelicolor deletion mutant for SCO0948 failed to produce a-mannosidase activity, confirming AM1 as the only alpha-mannosidase in S. coelicolor M145. Interestingly, the deletion mutant and a complementation strain produced lower levels of the antibiotics actinorhodin and undecylprodigiosin in glucose minimal media. The results indicate that AM1 as an alpha-mannosidase influences deglycosylation and antibiotic production in S. coelicolor M145. | - |
dc.publisher | HUMANA PRESS INC | - |
dc.relation.isPartOf | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | - |
dc.subject | MYCOBACTERIUM-TUBERCULOSIS | - |
dc.subject | DROSOPHILA-MELANOGASTER | - |
dc.subject | MACROLIDE ANTIBIOTICS | - |
dc.subject | GLYCOSYLATION SITES | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | GENE | - |
dc.subject | CLONING | - |
dc.subject | A3(2) | - |
dc.subject | BIOSYNTHESIS | - |
dc.subject | TRANSFERASE | - |
dc.title | Putative Role of a Streptomyces coelicolor-Derived alpha-Mannosidase in Deglycosylation and Antibiotic Production | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s12010-013-0635-y | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.172, no.3, pp.1639 - 1651 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000333246400039 | - |
dc.identifier.scopusid | 2-s2.0-84896313941 | - |
dc.citation.endPage | 1651 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 1639 | - |
dc.citation.title | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | - |
dc.citation.volume | 172 | - |
dc.contributor.affiliatedAuthor | Kim, Yun-Gon | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Streptomyces coelicolor | - |
dc.subject.keywordAuthor | alpha-Mannosidase | - |
dc.subject.keywordAuthor | Deglycosylation | - |
dc.subject.keywordAuthor | Antibiotic production | - |
dc.subject.keywordPlus | MYCOBACTERIUM-TUBERCULOSIS | - |
dc.subject.keywordPlus | DROSOPHILA-MELANOGASTER | - |
dc.subject.keywordPlus | MACROLIDE ANTIBIOTICS | - |
dc.subject.keywordPlus | GLYCOSYLATION SITES | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | A3(2) | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | TRANSFERASE | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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