Overexpression, crystallization and preliminary X-ray crystallographic analysis of a putative xylose isomerase from Bacteroides thetaiotaomicron
DC Field | Value | Language |
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dc.contributor.author | Cho, Jea-Won | - |
dc.contributor.author | Han, Byeong-Gu | - |
dc.contributor.author | Park, Sang Youn | - |
dc.contributor.author | Kim, Seung Jun | - |
dc.contributor.author | Kim, Myoung-Dong | - |
dc.contributor.author | Lee, Byung Il | - |
dc.date.available | 2018-05-09T13:58:33Z | - |
dc.date.created | 2018-04-17 | - |
dc.date.issued | 2013-10 | - |
dc.identifier.issn | 1744-3091 | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/11153 | - |
dc.description.abstract | Bacteroides thetaiotaomicron BT0793, a putative xylose isomerase, was overexpressed in Escherichia coli, purified and crystallized using polyethylene glycol monomethyl ether 550 as the precipitant. X-ray diffraction data were collected to 2.10 angstrom resolution at 100 Kusing synchrotron X-rays. The crystal was found to belong to space group P1, with unit-cell parameters a = 96.3, b = 101.7, c = 108.3 angstrom, alpha = 82.8, beta = 68.2, gamma = 83.0 degrees. The asymmetric unit contained eight subunits of xylose isomerase with a crystal volume per protein weight (V-M) of 2.38 angstrom(3) Da(-1) and a solvent content of 48.3%. | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.relation.isPartOf | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS | - |
dc.subject | GLUCOSE-ISOMERASE | - |
dc.subject | THERMOSTABILITY | - |
dc.title | Overexpression, crystallization and preliminary X-ray crystallographic analysis of a putative xylose isomerase from Bacteroides thetaiotaomicron | - |
dc.type | Article | - |
dc.identifier.doi | 10.1107/S1744309113023877 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, v.69, pp.1127 - 1130 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000325402900014 | - |
dc.identifier.scopusid | 2-s2.0-84885464894 | - |
dc.citation.endPage | 1130 | - |
dc.citation.startPage | 1127 | - |
dc.citation.title | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS | - |
dc.citation.volume | 69 | - |
dc.contributor.affiliatedAuthor | Park, Sang Youn | - |
dc.type.docType | Article | - |
dc.description.oadoiVersion | published | - |
dc.subject.keywordAuthor | Bacteroides thetaiotaomicron | - |
dc.subject.keywordAuthor | BT0793 | - |
dc.subject.keywordAuthor | glucose isomerase | - |
dc.subject.keywordAuthor | xylose isomerase | - |
dc.subject.keywordPlus | GLUCOSE-ISOMERASE | - |
dc.subject.keywordPlus | THERMOSTABILITY | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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