Functional analysis of the gene SCO1782 encoding Streptomyces hemolysin (S-hemolysin) in Streptomyces coelicolor M145
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rajesh, Thangamani | - |
dc.contributor.author | Jeon, Jong-Min | - |
dc.contributor.author | Kim, Yong-Hyun | - |
dc.contributor.author | Kim, Hyun-Joong | - |
dc.contributor.author | Yi, Da Hye | - |
dc.contributor.author | Park, Sung-Hee | - |
dc.contributor.author | Choi, Kwon-Young | - |
dc.contributor.author | Kim, Yun-Gon | - |
dc.contributor.author | Kim, Jaebum | - |
dc.contributor.author | Jung, Seunho | - |
dc.contributor.author | Park, Hyung-Yeon | - |
dc.contributor.author | Yang, Yung-Hun | - |
dc.date.available | 2018-05-09T14:01:27Z | - |
dc.date.created | 2018-04-17 | - |
dc.date.issued | 2013-09-01 | - |
dc.identifier.issn | 0041-0101 | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/11173 | - |
dc.description.abstract | In the process of evaluating the growth of Streptomyces coelicolor on rich media such as blood agar, we found that S. coelicolor a non-pathogenic, well-known antibiotic producer had the ability to grow and produce a prominent hemolytic zone. By comparing the growth with an agarase gene mutant of S. coelicolor, a similar prominent hemolytic zone was found to develop due to the organism's hemolytic activity. After the confirmation of hemolytic activity from S. coelicolor, the genome was searched for hemolysin-coding genes; consequently, SCO1782, SCO2534, and SCO3882 were identified, whose products were annotated as a putative, membrane, and hypothetical proteins, respectively. Functional characterization of all the recombinant proteins expressed in Escherichia coli BL21(DE3) revealed that only SC01782 exhibited hemolytic activity. This S. coelicolor protein, designated as S-hemolysin, showed sequence similarity toward hemolysins from Brachyspira hyodysenteriae (35%) and Mycobacterium tuberculosis (62%). Recombinant hemolysin exhibited activity against sheep blood erythrocytes and cytolytic activity against human fibroblast cells. Deletion of SC01782 resulted in complete loss of hemolysin activity in S. coelicolor. (C) 2013 Elsevier Ltd. All rights reserved. | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.relation.isPartOf | TOXICON | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | ANTIBIOTIC PRODUCTION | - |
dc.subject | ALPHA-HEMOLYSIN | - |
dc.subject | A3(2) | - |
dc.subject | GROWTH | - |
dc.subject | SOIL | - |
dc.subject | PATHOGENESIS | - |
dc.subject | EXPRESSION | - |
dc.subject | INFECTION | - |
dc.subject | CLONING | - |
dc.title | Functional analysis of the gene SCO1782 encoding Streptomyces hemolysin (S-hemolysin) in Streptomyces coelicolor M145 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.toxicon.2013.05.023 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | TOXICON, v.71, pp.159 - 165 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000322208100020 | - |
dc.identifier.scopusid | 2-s2.0-84879582307 | - |
dc.citation.endPage | 165 | - |
dc.citation.startPage | 159 | - |
dc.citation.title | TOXICON | - |
dc.citation.volume | 71 | - |
dc.contributor.affiliatedAuthor | Kim, Yun-Gon | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Streptomyces coelicolor | - |
dc.subject.keywordAuthor | S-hemolysim | - |
dc.subject.keywordAuthor | Hemolytic activity | - |
dc.subject.keywordAuthor | Cytolytic activity | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | ANTIBIOTIC PRODUCTION | - |
dc.subject.keywordPlus | ALPHA-HEMOLYSIN | - |
dc.subject.keywordPlus | A3(2) | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | SOIL | - |
dc.subject.keywordPlus | PATHOGENESIS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | CLONING | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Soongsil University Library 369 Sangdo-Ro, Dongjak-Gu, Seoul, Korea (06978)02-820-0733
COPYRIGHT ⓒ SOONGSIL UNIVERSITY, ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.