Screening and electrochemical detection of an antibiotic producing gene in bacteria on an integrated microchip
DC Field | Value | Language |
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dc.contributor.author | Han, Dawoon | - |
dc.contributor.author | Chand, Rohit | - |
dc.contributor.author | Shin, Ik-Soo | - |
dc.contributor.author | Kim, Yong-Sang | - |
dc.date.available | 2018-05-10T05:05:48Z | - |
dc.date.created | 2018-04-17 | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1759-9660 | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/12298 | - |
dc.description.abstract | We demonstrate the electrochemical determination of a secondary metabolite producing gene on an integrated microfluidic chip. The fabricated microchip was assembled with a continuous channel for polymerase chain reaction (PCR) and an electrochemical detector in order to achieve rapid and sensitive determination of the valC gene. valC is a gene responsible for producing antibiotic validamycin A in Streptomyces hygroscopicus. Biotin-conjugated primers amplified the valC gene. After the PCR, a DNA amplicon was analyzed in the electrochemical cell containing a streptavidin functionalized Au working electrode. The guanosine present in the DNA amplicon released electrons upon electrochemical oxidation at 0.93 V and the peak current linearly increased with the concentration of the captured DNA amplicon. The fabricated chip successfully amplified and detected the valC gene as low as 30 pg mu L-1 resulting in a sensitive, portable and integrated DNA analysis for a secondary metabolite. | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.relation.isPartOf | ANALYTICAL METHODS | - |
dc.subject | REAL-TIME PCR | - |
dc.subject | CAPILLARY-ELECTROPHORESIS | - |
dc.subject | DNA AMPLIFICATION | - |
dc.subject | FLUORESCENCE DETECTION | - |
dc.subject | VALIDAMYCIN-A | - |
dc.subject | CHIP | - |
dc.subject | SURFACE | - |
dc.subject | SENSOR | - |
dc.subject | POLY(DIMETHYLSILOXANE) | - |
dc.subject | FABRICATION | - |
dc.title | Screening and electrochemical detection of an antibiotic producing gene in bacteria on an integrated microchip | - |
dc.type | Article | - |
dc.identifier.doi | 10.1039/c3ay41321g | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ANALYTICAL METHODS, v.5, no.23, pp.6814 - 6820 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000326946700040 | - |
dc.identifier.scopusid | 2-s2.0-84887422732 | - |
dc.citation.endPage | 6820 | - |
dc.citation.number | 23 | - |
dc.citation.startPage | 6814 | - |
dc.citation.title | ANALYTICAL METHODS | - |
dc.citation.volume | 5 | - |
dc.contributor.affiliatedAuthor | Shin, Ik-Soo | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | REAL-TIME PCR | - |
dc.subject.keywordPlus | CAPILLARY-ELECTROPHORESIS | - |
dc.subject.keywordPlus | DNA AMPLIFICATION | - |
dc.subject.keywordPlus | FLUORESCENCE DETECTION | - |
dc.subject.keywordPlus | VALIDAMYCIN-A | - |
dc.subject.keywordPlus | CHIP | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordPlus | POLY(DIMETHYLSILOXANE) | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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