Crystallization and preliminary X-ray crystallographic analysis of Thermotoga maritima CheA P3-P4-P5 domains in complex with CheW
DC Field | Value | Language |
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dc.contributor.author | Park, SangYoun | - |
dc.contributor.author | Kim, Keon Young | - |
dc.contributor.author | Kim, Sunmin | - |
dc.contributor.author | Crane, Brian R. | - |
dc.date.available | 2018-05-10T05:19:23Z | - |
dc.date.created | 2018-04-17 | - |
dc.date.issued | 2012-06 | - |
dc.identifier.issn | 1744-3091 | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/12410 | - |
dc.description.abstract | The CheACheW complex plays a key role in bacterial chemotaxis signal transduction by initiating phosphotransfer to response regulators via coupling to the chemoreceptors. CheA (P3-P4-P5 domains) and CheW from Thermotoga maritima were overexpressed in Escherichia coli and crystallized as a complex at 298 K using ammonium dihydrogen phosphate as a precipitant. X-ray diffraction data were collected to 8 angstrom resolution at 100 K using synchrotron radiation. The crystal belonged to space group I222 or I212121, with unit-cell parameters a = 184.2, b = 286.4, c = 327.7 angstrom. The asymmetric unit may contain six to ten CheACheW molecules. | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.relation.isPartOf | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS | - |
dc.subject | BACTERIAL CHEMOTAXIS | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | PROTEIN CRYSTALS | - |
dc.subject | KINASE | - |
dc.subject | CRYOCRYSTALLOGRAPHY | - |
dc.subject | DIFFRACTION | - |
dc.title | Crystallization and preliminary X-ray crystallographic analysis of Thermotoga maritima CheA P3-P4-P5 domains in complex with CheW | - |
dc.type | Article | - |
dc.identifier.doi | 10.1107/S174430911201826X | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, v.68, pp.713 - 715 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000305073600025 | - |
dc.identifier.scopusid | 2-s2.0-84862191447 | - |
dc.citation.endPage | 715 | - |
dc.citation.startPage | 713 | - |
dc.citation.title | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS | - |
dc.citation.volume | 68 | - |
dc.contributor.affiliatedAuthor | Park, SangYoun | - |
dc.type.docType | Article | - |
dc.description.oadoiVersion | published | - |
dc.subject.keywordAuthor | CheA | - |
dc.subject.keywordAuthor | histidine kinases | - |
dc.subject.keywordAuthor | CheW | - |
dc.subject.keywordAuthor | coupling proteins | - |
dc.subject.keywordAuthor | bacterial chemotaxis | - |
dc.subject.keywordAuthor | signal transduction | - |
dc.subject.keywordAuthor | Thermotoga maritima | - |
dc.subject.keywordPlus | BACTERIAL CHEMOTAXIS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | PROTEIN CRYSTALS | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | CRYOCRYSTALLOGRAPHY | - |
dc.subject.keywordPlus | DIFFRACTION | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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