Structural insight into the low affinity between Thermotoga maritima CheA and CheB compared to their Escherichia coli/Salmonella typhimurium counterparts
- Authors
- Park, SangYoun; Crane, Brian R.
- Issue Date
- Nov-2011
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Bacterial chemotaxis; CheB; CheA; Thermotoga maritima; Protein-protein interaction
- Citation
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.49, no.4, pp.794 - 800
- Journal Title
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
- Volume
- 49
- Number
- 4
- Start Page
- 794
- End Page
- 800
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/13543
- DOI
- 10.1016/j.ijbiomac.2011.07.012
- ISSN
- 0141-8130
- Abstract
- CheA-mediated CheB phosphorylation and the subsequent CheB-mediated demethylation of the chemoreceptors are important steps required for the bacterial chemotactic adaptation response. Although Escherichia coli CheB has been reported to interact with CheA competitively against CheY, we have observed that Thermotoga maritima CheB has no detectable CheA-binding. By determining the CheY-like domain crystal structure of T. maritima CheB, and comparing against the T. maritima CheY and Salmonella typhimurium CheB structures, we propose that the two consecutive glutamates in the beta 4/alpha 4 loop of T. maritima CheB that is absent in T. maritima CheY and in E. coli/S. typhimurium CheB may be one factor contributing to the low CheA affinity. (C) 2011 Elsevier B.V. All rights reserved.
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