Inhibitory effect of Sanguisorba officinalis ethanol extract on NO and PGE(2) production is mediated by suppression of NF-kappa B and AP-1 activation signaling cascade
- Authors
- Yu, Tao; Lee, Yong Jin; Yang, Hyun Mo; Han, Soryu; Kim, Jae Hun; Lee, Yoonsuk; Kim, Changhyuk; Han, Moon Hi; Kim, Mi-Yeon; Lee, Jaehwi; Cho, Jae You
- Issue Date
- 8-Mar-2011
- Publisher
- ELSEVIER IRELAND LTD
- Keywords
- Sanguisorba officinalis; Inflammatory mediators; NF-kappa B; AP-1 translocation; Src activation
- Citation
- JOURNAL OF ETHNOPHARMACOLOGY, v.134, no.1, pp.11 - 17
- Journal Title
- JOURNAL OF ETHNOPHARMACOLOGY
- Volume
- 134
- Number
- 1
- Start Page
- 11
- End Page
- 17
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/13705
- DOI
- 10.1016/j.jep.2010.08.060
- ISSN
- 0378-8741
- Abstract
- Aim of the study: Sanguisorba officinalis, a well known valuable medicinal plant in Korea, China and Japan used traditionally for the treatment of inflammatory and metabolic diseases such as diarrhea, chronic intestinal infections, duodenal ulcers, and bleeding. Recent studies have revealed that its aqueous or ethanolic extracts exhibit a variety of pharmacological activities such as anti-oxidative, anti-cancer, anti-lipid peroxidation, anti-atherogenic, and vasorelaxant effects. Systematic studies on the anti-inflammatory effect of this plant and its molecular mechanisms have not yet been fully investigated. Ethanol extract of Sanguisorba officinalis (So-EE) the lipopolysaccharide (LPS)-stimulated macrophages and production of inflammatory mediators were employed to assess these properties. Results: So-EE significantly suppressed the production of nitric oxide (NO) and prostaglandin (PG) E-2 from LPS-activated RAW264.7 cells and peritoneal macrophages in a dose-dependent manner. This extract effectively diminished the mRNA levels of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2, implying that the blockade is generated at the transcriptional level. So-EE strongly blocked the activation and translocation of NF-kappa B and AP-1 by suppressing the upstream kinases including inhibitor of kappa B alpha (I kappa B alpha), I kappa B alpha kinase (IKK), Akt (protein kinase B), phosphoinositide-dependent kinase 1 (PDK1), p85/phosphoinositide-3-kinase (PI3K), and mitogen activated protein kinase (MAPK) such as extracellular signal-regulated kinase (ERK), p38, and c-Jun N-terminal kinase (JNK). Moreover, So-EE suppressed the phosphorylation of Src, its kinase activity, and complex formation between Src and p85. Conclusion: This study suggests that So-EE has a potent anti-inflammatory activity mediated by NF-kappa B, and AP-1 inhibitory properties linked to the suppression of Src and MAPK activation. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Natural Sciences > School of Systems and Biomedical Science > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/13705)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.