Src-mediated regulation of inflammatory responses by actin polymerization
DC Field | Value | Language |
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dc.contributor.author | Kim, Joo Young | - |
dc.contributor.author | Lee, Yong Gyu | - |
dc.contributor.author | Kim, Mi-Yeon | - |
dc.contributor.author | Byeon, Se Eun | - |
dc.contributor.author | Rhee, Man Hee | - |
dc.contributor.author | Park, Jongsun | - |
dc.contributor.author | Katz, David R. | - |
dc.contributor.author | Chain, Benjamin M. | - |
dc.contributor.author | Cho, Jae Youl | - |
dc.date.available | 2018-05-10T14:14:08Z | - |
dc.date.created | 2018-04-17 | - |
dc.date.issued | 2010-02-01 | - |
dc.identifier.issn | 0006-2952 | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/14806 | - |
dc.description.abstract | Although the role of the actin cytoskeleton has become increasingly elucidated, the role of actin polymerization in inflammatory processes remains poorly understood. Here, we examine the role of the actin cytoskeleton during LPS-mediated inflammatory events in RAW264.7 cells and peritoneal macrophages. We observed that actin cytoskeleton disruption by cytochalasin B and siRNA to cytoplasmic actin strongly down-regulated LPS-mediated inflammatory responses such as NO production, PGE(2) release, and TNF-alpha secretion. Actin cytoskeleton disruption by cytochalasin B down-regulated a series of signaling cascades including P13K, Akt, and IKK, but not MAPKs, necessary for NF-kappa B activation without down-regulating total forms of the proteins as assessed by measuring their phosphorylation levels. In particular, cytochalasin B significantly inhibited LPS-induced both phosphorylation and kinase activity of Src without altering total level, implying that Src may be a potential pharmacological target of actin cytoskeleton rearrangement. Moreover, the direct association of Src with actin was actin polymerization-dependent according to immunoprecipitation analysis performed with a GFP-actin wild type and HA-tagged Src. Therefore, our data suggest that actin cytoskeleton rearrangement may be a key event during the regulation of inflammatory responses that modulates the activity of Src and its downstream signaling molecules. (C) 2009 Elsevier Inc. All rights reserved. | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.relation.isPartOf | BIOCHEMICAL PHARMACOLOGY | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE-1 | - |
dc.subject | NITRIC-OXIDE PRODUCTION | - |
dc.subject | TYROSINE PHOSPHORYLATION | - |
dc.subject | MYELOMONOCYTIC CELLS | - |
dc.subject | SIGNAL-TRANSDUCTION | - |
dc.subject | DEPENDENT PATHWAY | - |
dc.subject | LIQUID CULTURE | - |
dc.subject | NO PRODUCTION | - |
dc.subject | YEAST ACTIN | - |
dc.title | Src-mediated regulation of inflammatory responses by actin polymerization | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bcp.2009.09.016 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL PHARMACOLOGY, v.79, no.3, pp.431 - 443 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000273694900015 | - |
dc.identifier.scopusid | 2-s2.0-70450223330 | - |
dc.citation.endPage | 443 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 431 | - |
dc.citation.title | BIOCHEMICAL PHARMACOLOGY | - |
dc.citation.volume | 79 | - |
dc.contributor.affiliatedAuthor | Kim, Mi-Yeon | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Actin cytoskeleton | - |
dc.subject.keywordAuthor | Inflammation | - |
dc.subject.keywordAuthor | Cytochalasin B | - |
dc.subject.keywordAuthor | Src kinase | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE-1 | - |
dc.subject.keywordPlus | NITRIC-OXIDE PRODUCTION | - |
dc.subject.keywordPlus | TYROSINE PHOSPHORYLATION | - |
dc.subject.keywordPlus | MYELOMONOCYTIC CELLS | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | DEPENDENT PATHWAY | - |
dc.subject.keywordPlus | LIQUID CULTURE | - |
dc.subject.keywordPlus | NO PRODUCTION | - |
dc.subject.keywordPlus | YEAST ACTIN | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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