In Situ Single Cell Monitoring by Isocyanide-Functionalized Ag and Au Nanoprobe-Based Raman Spectroscopy
- Authors
- Lee, So Yeong; Jang, Soo Hwa; Cho, Myung-Haing; Kim, Young Min; Cho, Keun Chang; Ryu, Pan Dong; Gong, Myoung-Seon; Joo, Sang-Woo
- Issue Date
- Sep-2009
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- In situ cellular imaging; nanoparticles; surface modification; Raman spectroscopy; cell monitoring
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.19, no.9, pp.904 - 910
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 19
- Number
- 9
- Start Page
- 904
- End Page
- 910
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/15778
- DOI
- 10.4014/jmb.0901.009
- ISSN
- 1017-7825
- Abstract
- The development of effective cellular imaging requires a specific labeling method for targeting, tracking, and monitoring cellular/molecular events in the living organism. For this purpose, we studied the cellular uptake of isocyanide-functionalized silver and gold nanoparticles by surface-enhanced Raman scattering (SERS). Inside a single mammalian cell, we could monitor the intracellular behavior of such nanoparticles by measuring the SERS spectra. The NC stretching band appeared clearly at similar to 2,100 cm(-1) in the well-isolated spectral region from many organic constituents between 300 and 1,700 or 2,800 and 3,600 cm(-1). The SERS marker band at similar to 2,100 cm(-1) could be used to judge the location of the isocyanide-functionalized nanoparticles inside the cell without much spectral interference from other cellular constituents. Our results demonstrate that isocyanide-modified silver or gold nanoparticle-based SERS may have high potential for monitoring and imaging the biological processes at the single cell level.
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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