Production and structural features of a water-soluble polysaccharide from a mutant strain of Agrobacterium sp.
- Authors
- Yoon, Shinsook; Kim, Mi-Kyoung; Lee, In-Young; Yun, Mikyung; Shin, Jeong E. Nam
- Issue Date
- Nov-2008
- Publisher
- KOREAN SOC INDUSTRIAL ENGINEERING CHEMISTRY
- Keywords
- Water-soluble polysaccharide; Agrobacterium sp.; Mutant strain; Production; Structure
- Citation
- JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, v.14, no.6, pp.759 - 764
- Journal Title
- JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
- Volume
- 14
- Number
- 6
- Start Page
- 759
- End Page
- 764
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/16775
- DOI
- 10.1016/j.jiec.2008.06.001
- ISSN
- 1226-086X
- Abstract
- We have developed a mutant strain derived from Agrobacterium sp. ATCC 31750, which produces a water-soluble polysaccharide having potential utility to the food, feed, pharmaceutical and cosmetic industries. A high concentration of product (15 g/L) is obtained by 48 h cultivation of the mutant strain under optimized fermentation conditions. The water-soluble polysaccharide obtained from cultures of the mutant strain beta82 has Glc:Man:Gal in approximate molar ratios of 5.8:6.7: 1.0. The molecular weight of the polysaccharide was determined to be approximately 1000 kDa by HPSEC analysis. Linkage analysis contained 3-Glcp, 3-Manp, terminal Glcp and terminal Manp, as well as a small proportion of 3- and 3,4-Galp, and 4,6-Manp residues. Based on analyses using FT-IR and (13)C NMR spectrometers, most glycosidic bonds joining these sugar residues are of the (x-type, and acetyl groups are apparently attached to the polymer chain at random. (C) 2008 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
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