Mutational analysis of human tumor necrosis factor-alpha
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, HC | - |
dc.contributor.author | Cho, KH | - |
dc.date.available | 2018-05-10T17:41:16Z | - |
dc.date.created | 2018-04-17 | - |
dc.date.issued | 2005-01 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/19408 | - |
dc.description.abstract | To understand the structure-function relationship of human tumor necrosis factor-alpha ( TNF-alpha), mutational analysis was carried out on the lower regions ( regions 1-6) of the molecule. The muteins were prepared as a soluble form by using a chaperonin co-expression system and the cytotoxic activities of the purified muteins were evaluated on TNF-sensitive murine. fibrosarcoma L929 cells. Three regions ( regions 1, 2 & 4) were found where mutations significantly influenced the bioactivity. In region 1 ( residues 1-10), the number of deleted residues and the positioning of positive charges are important to achieve a maximum activity and in region 4 ( residues 84-88), introduction of charged residues in one of the positions 86-88 significantly increased the cytotoxic activity. On the other hand, any mutation introduced in region 2 ( residues 37-41) had a deleterious effect. The present study provides a structural basis for the design of highly potent TNF-alpha as a therapeutic agent. | - |
dc.publisher | SPRINGER | - |
dc.relation.isPartOf | BIOTECHNOLOGY LETTERS | - |
dc.subject | MONOCLONAL-ANTIBODIES | - |
dc.subject | ANTITUMOR-ACTIVITY | - |
dc.subject | CRYSTAL-STRUCTURE | - |
dc.subject | TNF RECEPTOR | - |
dc.subject | CELL-LINES | - |
dc.subject | TOXICITY | - |
dc.subject | BINDING | - |
dc.subject | RESOLUTION | - |
dc.subject | MUTEINS | - |
dc.subject | INVIVO | - |
dc.title | Mutational analysis of human tumor necrosis factor-alpha | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s10529-004-6937-y | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY LETTERS, v.27, no.2, pp.107 - 112 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000226971300007 | - |
dc.identifier.scopusid | 2-s2.0-14644408128 | - |
dc.citation.endPage | 112 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 107 | - |
dc.citation.title | BIOTECHNOLOGY LETTERS | - |
dc.citation.volume | 27 | - |
dc.contributor.affiliatedAuthor | Shin, HC | - |
dc.contributor.affiliatedAuthor | Cho, KH | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | cytotoxicity | - |
dc.subject.keywordAuthor | muteins | - |
dc.subject.keywordAuthor | receptor binding | - |
dc.subject.keywordAuthor | structure-function relationship | - |
dc.subject.keywordAuthor | tumor necrosis factor-alpha | - |
dc.subject.keywordPlus | MONOCLONAL-ANTIBODIES | - |
dc.subject.keywordPlus | ANTITUMOR-ACTIVITY | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | TNF RECEPTOR | - |
dc.subject.keywordPlus | CELL-LINES | - |
dc.subject.keywordPlus | TOXICITY | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | RESOLUTION | - |
dc.subject.keywordPlus | MUTEINS | - |
dc.subject.keywordPlus | INVIVO | - |
dc.description.journalRegisteredClass | scopus | - |
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