Formation of an alpha-helix in human tumor necrosis factor-alpha by guanidine hydrochloride-induced unfolding
DC Field | Value | Language |
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dc.contributor.author | Jeong, WJ | - |
dc.contributor.author | Shin, HC | - |
dc.date.available | 2018-05-10T18:05:51Z | - |
dc.date.created | 2018-04-17 | - |
dc.date.issued | 2004-02-29 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/20022 | - |
dc.description.abstract | Human tumor necrosis factor-alpha (TNF-alpha) is a trimeric protein consisting primarily of beta-sheet. GdnHCl-induced unfolding of TNF-alpha was investigated at room temperature by circular dichroism (CD) and size exclusion chromatography. The secondary and tertiary structure of TNF-alpha persisted up to 0.9N GdnHCl regardless of incubation time, but, in the range of 1.2 N to 2.1 N GdnHCl, there was loss of tertiary structure accompanied by the formation of an alpha-helix, as revealed by far- and near-UV CD spectra. The structural changes occurred gradually in 1.2 and 2.1 N GdnHCl, but were rapid in 1.5 and 1.8 N GdnHCl. The GdnHCl-induced state of TNF-alpha is an unfolded, a-helical aggregate of about 130 monomers, as shown by size exclusion chromatography. We suggest the most likely pathway for the transition from beta-sheet to alpha-helix. | - |
dc.publisher | SPRINGER-VERLAG SINGAPORE PTE LTD | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.subject | PANCREATIC TRYPSIN-INHIBITOR | - |
dc.subject | FOLDING INITIATION SITES | - |
dc.subject | INDUCED DENATURATION | - |
dc.subject | MOLTEN GLOBULE | - |
dc.subject | PEPTIDE MODELS | - |
dc.subject | PROTEIN | - |
dc.subject | MYOGLOBIN | - |
dc.subject | INDUCTION | - |
dc.subject | SEQUENCE | - |
dc.subject | MUTANT | - |
dc.title | Formation of an alpha-helix in human tumor necrosis factor-alpha by guanidine hydrochloride-induced unfolding | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.17, no.1, pp.62 - 66 | - |
dc.identifier.kciid | ART000957719 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000189388600011 | - |
dc.identifier.scopusid | 2-s2.0-4344623932 | - |
dc.citation.endPage | 66 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 62 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 17 | - |
dc.contributor.affiliatedAuthor | Shin, HC | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | circular dichroism | - |
dc.subject.keywordAuthor | guanidine hydrochloride | - |
dc.subject.keywordAuthor | induction of alpha-helix | - |
dc.subject.keywordAuthor | protein unfolding | - |
dc.subject.keywordAuthor | tumor necrosis factor-alpha | - |
dc.subject.keywordPlus | PANCREATIC TRYPSIN-INHIBITOR | - |
dc.subject.keywordPlus | FOLDING INITIATION SITES | - |
dc.subject.keywordPlus | INDUCED DENATURATION | - |
dc.subject.keywordPlus | MOLTEN GLOBULE | - |
dc.subject.keywordPlus | PEPTIDE MODELS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | MYOGLOBIN | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | MUTANT | - |
dc.description.journalRegisteredClass | scopus | - |
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