Quantitative characterization of intact sialylated O-glycans with MALDI-MS for protein biotherapeutics
DC Field | Value | Language |
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dc.contributor.author | Hwang, C.-H. | - |
dc.contributor.author | Park, H.-M. | - |
dc.contributor.author | Park, H.-G. | - |
dc.contributor.author | Ahn, D.-H. | - |
dc.contributor.author | Kim, S.-M. | - |
dc.contributor.author | Ko, B.J. | - |
dc.contributor.author | Kim, Y.H. | - |
dc.contributor.author | Yang, Y.-H. | - |
dc.contributor.author | Kim, Y.-G. | - |
dc.date.available | 2019-03-13T01:41:47Z | - |
dc.date.created | 2018-09-12 | - |
dc.date.issued | 2018-07 | - |
dc.identifier.issn | 0256-1115 | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/31508 | - |
dc.description.abstract | For validating O-glycosylation of protein biotherapeutics, we presented a quantitative O-glycomics method which is based on the neutralization of sialic acids, the specific release of O-glycans, and the introduction of permanent positive charge followed by quantitative MALDI-MS analysis. This method shows excellent technical reproducibility, linearity and sensitivity. In addition, it enables the quantification of intact O-glycans with minimal degradation or loss of sialic acids on these glycans compared to a conventional HPLC-based method. We then applied this method to quantitatively characterize O-glycans present on Etanercept. The analysis showed the relative abundances of mono- and di-sialylated core 1 O-glycans - were 79.3±0.8% and 17.3±1.4%, respectively. This glycomics technology could allow for the reliable quantitative analysis of intact O-glycans from glycoproteins and may contribute to validation of O-glycosylation protein biotherapeutics in the pharmaceutical industry. © 2018, Korean Institute of Chemical Engineers, Seoul, Korea. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Springer New York LLC | - |
dc.relation.isPartOf | Korean Journal of Chemical Engineering | - |
dc.title | Quantitative characterization of intact sialylated O-glycans with MALDI-MS for protein biotherapeutics | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s11814-018-0058-0 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Korean Journal of Chemical Engineering, v.35, no.7, pp.1462 - 1467 | - |
dc.identifier.kciid | ART002355416 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000437111400008 | - |
dc.identifier.scopusid | 2-s2.0-85047118996 | - |
dc.citation.endPage | 1467 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1462 | - |
dc.citation.title | Korean Journal of Chemical Engineering | - |
dc.citation.volume | 35 | - |
dc.contributor.affiliatedAuthor | Kim, Y.-G. | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordAuthor | Chemical Derivatization | - |
dc.subject.keywordAuthor | MALDI-MS | - |
dc.subject.keywordAuthor | O-glycan | - |
dc.subject.keywordAuthor | Protein Biotherapeutics | - |
dc.subject.keywordAuthor | Quantitative Analysis | - |
dc.subject.keywordPlus | FLIGHT MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | THERAPEUTIC PROTEINS | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
dc.subject.keywordPlus | GLYCOMICS | - |
dc.subject.keywordPlus | DERIVATIZATION | - |
dc.subject.keywordPlus | PERMETHYLATION | - |
dc.subject.keywordPlus | ENHANCEMENT | - |
dc.description.journalRegisteredClass | scie | - |
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