Engineering of artificial microbial consortia of Ralstonia eutropha and Bacillus subtilis for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer production from sugarcane sugar without precursor feeding
- Authors
- Bhatia, Shashi Kant; Yoon, Jeong-Jun; Kim, Hyun-Joong; Hong, Ju Won; Hong, Yoon Gi; Song, Hun-Seok; Moon, Yu-Mi; Jeon, Jong-Min; Kim, Yun-Gon; Yang, Yung-Hun
- Issue Date
- Feb-2018
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Ralstonia eutropha; Polyhydroxyalkonate; Co-culture; Poly(3-hydroxybutyrate-co-3-hydroxyvalerate); Consortia
- Citation
- BIORESOURCE TECHNOLOGY, v.257, pp.92 - 101
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 257
- Start Page
- 92
- End Page
- 101
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/32027
- DOI
- 10.1016/j.biortech.2018.02.056
- ISSN
- 0960-8524
- Abstract
- Ralstonia eutropha is a well-known microbe reported for polyhydroxyalkonate (PHA) production, and unable to utilize sucrose as carbon source. Two strains, Ralstonia eutropha H16 and Ralstonia eutropha 5119 were co-cultured with sucrose hydrolyzing microbes (Bacillus subtilis and Bacillus amyloliquefaciens) for PHA production. Co-culture of B. subtilis: R. eutropha 5119 (BS:RE5) resulted in best PHA production (45% w/w dcw). Optimization of the PHA production process components through response surface resulted in sucrose: NH4Cl:B. subtilis:R. eutropha (3.0:0.17:0.10:0.190). Along with the hydrolysis of sucrose, B. subtilis also ferments sugars into organic acid (propionic acid), which acts as a precursor for HV monomer unit. Microbial consortia of BS:RE5 when cultured in optimized media led to the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV) with 66% w/w of dcw having 16 mol% HV fraction. This co-culture strategy overcomes the need for metabolic engineering of R. eutropha for sucrose utilization, and addition of precursor for copolymer production.
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Collections - College of Engineering > Department of Chemical Engineering > 1. Journal Articles
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