Trichosanthes tricuspidata Lour. Methanol Extract Exhibits Anti-Inflammatory Activity by Targeting Syk, Src, and IRAK1 Kinase Activity
DC Field | Value | Language |
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dc.contributor.author | Ahuja A. | - |
dc.contributor.author | Jeong D. | - |
dc.contributor.author | Kim M.-Y. | - |
dc.contributor.author | Cho J.Y. | - |
dc.date.available | 2020-03-18T02:20:07Z | - |
dc.date.created | 2020-03-18 | - |
dc.date.issued | 2019-12 | - |
dc.identifier.issn | 1741-427X | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/35554 | - |
dc.description.abstract | Trichosanthes tricuspidata Lour., also known as T. palmata Roxb, T. bracteata Lam., T. puber Blume, and Modecca bracteata, is a vine belonging to the Cucurbitaceae family (English name: redball snake gourd). Distributed in China, South and East Asia, and tropical Australia, it has been traditionally used as a medicinal plant for its antifever, laxative, anthelmintic properties and for migraine treatment. In this paper, we examined the effects of Trichosanthes tricuspidata Lour. ethanol extract (Tt-ME) in vitro and in vivo. To confirm the effects of Tt-ME on inflammatory responses, we conducted experimental analyses including level of nitric oxide (NO) production, RT-PCR, and immunoblotting and using a HCl/EtOH-induced gastritis animal model. Tt-ME attenuated the release of NO and decreased mRNA levels of inducible NO synthase (iNOS), TNF-α, and IL-6 in lipopolysaccharide- (LPS-) induced macrophages in a concentration-dependent manner. Tt-ME time-dependently suppressed nuclear translocation of nuclear factor kappa B (NF-B) subunits p50 and p65, activator protein (AP-1) subunits c-Fos and c-Jun, and STAT3 transcriptional activity by inhibiting nuclear translocation of p50, p65, c-Fos, c-Jun, and STAT3. Tt-ME significantly downregulated NF-B, MAPK, and JAK2 signaling by targeting Syk, Src, and IRAK1 protein kinases. Furthermore, matrix metalloproteinase-9 (MMP-9) expression and cell migration were observed to be downregulated by Tt-ME in LPS-activated macrophages. In vivo studies on Tt-ME also produced similar trends in Hcl/EtOH-induced gastritis mouse models by inhibiting proinflammatory cytokines and the inflammatory signaling pathway. Our results strongly suggest that Tt-ME exerted anti-inflammatory activity in LPS-stimulated macrophages and mouse models of acute inflammatory disease. © 2019 Akash Ahuja et al. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Hindawi Limited | - |
dc.relation.isPartOf | Evidence-based Complementary and Alternative Medicine | - |
dc.title | Trichosanthes tricuspidata Lour. Methanol Extract Exhibits Anti-Inflammatory Activity by Targeting Syk, Src, and IRAK1 Kinase Activity | - |
dc.type | Article | - |
dc.identifier.doi | 10.1155/2019/6879346 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Evidence-based Complementary and Alternative Medicine, v.2019 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000522249300002 | - |
dc.identifier.scopusid | 2-s2.0-85077458090 | - |
dc.citation.title | Evidence-based Complementary and Alternative Medicine | - |
dc.citation.volume | 2019 | - |
dc.contributor.affiliatedAuthor | Kim M.-Y. | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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