Noncovalent tethering of nucleic acid aptamer on DNA nanostructure for targeted photo/chemo/gene therapies
- Authors
- Shim, Gayong; Park, Jinwon; Kim, Mi-Gyeong; Yang, Geon; Lee, Yeon; Oh, Yu-Kyoung
- Issue Date
- Feb-2020
- Publisher
- ELSEVIER
- Keywords
- DNA nanostructure; Polydopamine shell; Targeted therapy; Noncovalent DNA aptamer anchoring; Versatile cargoes
- Citation
- NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, v.24
- Journal Title
- NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
- Volume
- 24
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/39950
- DOI
- 10.1016/j.nano.2019.102053
- ISSN
- 1549-9634
- Abstract
- Here we report various therapeutic cargo-loadable DNA nanostructures that are shelled in polydopamine and noncovalently tethered with cancer cell-targeting DNA aptamers. Initial DNA nanostructure was formed by rolling-circle amplification and condensation with Mu peptides. This DNA nanostructure was loaded with an antisense oligonucleotide, a photosensitizer, or an anticancer chemotherapeutic drug. Each therapeutic agent-loaded DNA nanostructure was then shelled with polydopamine (PDA), and noncovalently decorated with a poly adenine-tailed nucleic acid aptamer (PA) specific for PTK7 receptor, resulting in PA-tethered and PDA-shelled DNA nanostructure (PA/PDN). PDA coating shell enabled photothermal therapy. In the cells overexpressing PTK7 receptor, photosensitizer-loaded PA/PDN showed greater photodynamic activity. Doxontbicin-loaded PA/PDN exerted higher anticancer activity than the other groups. Antisense oligonucleotide-loaded PA/PDN provided selective reduction of target proteins compared with other groups. Our results suggest that the PA-tethered and PDA-shelled DNA nanostructures could enable the specific receptor-targeted phototherapy, chemotherapy, and gene therapy against cancer cells. (C) 2019 Elsevier Inc. All rights reserved.
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Collections - College of Natural Sciences > School of Systems and Biomedical Science > 1. Journal Articles
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