The conserved microRNA miR-8-3p coordinates the expression of V-ATPase subunits to regulate ecdysone biosynthesis for Drosophila metamorphosis
DC Field | Value | Language |
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dc.contributor.author | Lim, Do-Hwan | - |
dc.contributor.author | Lee, Seungjae | - |
dc.contributor.author | Choi, Min-Seok | - |
dc.contributor.author | Han, Jee Yun | - |
dc.contributor.author | Seong, Youngmo | - |
dc.contributor.author | Na, Dokyun | - |
dc.contributor.author | Kwon, Young-Soo | - |
dc.contributor.author | Lee, Young Sik | - |
dc.date.available | 2021-03-03T01:40:11Z | - |
dc.date.created | 2021-03-03 | - |
dc.date.issued | 2020-05 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/40308 | - |
dc.description.abstract | The steroid hormone ecdysone is the central regulator of insect metamorphosis, during which a growing, immature larva is remodeled, through pupal stages, to a reproductive adult. However, the underlying mechanisms of ecdysone-mediated metamorphosis remain to be fully elucidated. Here, we identified metamorphosis-associated microRNAs (miRNAs) and their potential targets by cross-linking immunoprecipitation coupled with deep sequencing of endogenous Argonaute 1 protein in Drosophila. Interestingly, miR-8-3p targeted five Vha genes encoding distinct subunits of vacuolar H+-ATPase (V-ATPase), which has a vital role in the organellar acidification. The expression of ecdysone-responsive miR-8-3p is normally downregulated during Drosophila metamorphosis, but temporary overexpression of miR-8-3p in the whole body at the end of larval development led to defects in metamorphosis and survival, hallmarks of aberrant ecdysone signaling. In addition, miR-8-3p was expressed in the prothoracic gland (PG), which produces and releases ecdysone in response to prothoracicotropic hormone (PTTH). Notably, overexpression of miR-8-3p or knockdown of its Vha targets in the PG resulted in larger than normal, ecdysone-deficient larvae that failed to develop into the pupal stage but could be rescued by ecdysone feeding. Moreover, these animals showed defective PTTH signaling with a concomitant decrease in the expression of ecdysone biosynthetic genes. We also demonstrated that the regulatory network between the conserved miR-8-3p/miR-200 family and V-ATPase was functional in human cells. Consequently, our data indicate that the coordinated regulation of V-ATPase subunits by miR-8-3p is involved in Drosophila metamorphosis by controlling the ecdysone biosynthesis. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.relation.isPartOf | FASEB JOURNAL | - |
dc.title | The conserved microRNA miR-8-3p coordinates the expression of V-ATPase subunits to regulate ecdysone biosynthesis for Drosophila metamorphosis | - |
dc.type | Article | - |
dc.identifier.doi | 10.1096/fj.201901516R | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | FASEB JOURNAL, v.34, no.5, pp.6449 - 6465 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000521550600001 | - |
dc.citation.endPage | 6465 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 6449 | - |
dc.citation.title | FASEB JOURNAL | - |
dc.citation.volume | 34 | - |
dc.contributor.affiliatedAuthor | Lim, Do-Hwan | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordAuthor | Ago1 | - |
dc.subject.keywordAuthor | CLIP-seq | - |
dc.subject.keywordAuthor | ecdysone | - |
dc.subject.keywordAuthor | metamorphosis | - |
dc.subject.keywordAuthor | microRNA | - |
dc.subject.keywordAuthor | V-ATPase | - |
dc.subject.keywordPlus | PROTHORACIC GLAND | - |
dc.subject.keywordPlus | H+-ATPASE | - |
dc.subject.keywordPlus | MESENCHYMAL TRANSITION | - |
dc.subject.keywordPlus | VACUOLAR ATPASE | - |
dc.subject.keywordPlus | MIR-200 FAMILY | - |
dc.subject.keywordPlus | INDUCIBLE GENE | - |
dc.subject.keywordPlus | EARLY PUFF | - |
dc.subject.keywordPlus | BODY-SIZE | - |
dc.subject.keywordPlus | ENCODES 2 | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Life Sciences & Biomedicine - Other Topics | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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