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Capturing the interactome of newly transcribed RNA
- Authors
- Bao, Xichen; Guo, Xiangpeng; Yin, Menghui; Tariq, Muqddas; Lai, Yiwei; Kanwal, Shahzina; Zhou, Jiajian; Li, Na; Lv, Yuan; Pulido-Quetglas, Carlos; Wang, Xiwei; Ji, Lu; Khan, Muhammad J.; Zhu, Xihua; Luo, Zhiwei; Shao, Changwei; Lim, Do-Hwan; Liu, Xiao; Li, Nan; Wang, Wei; He, Minghui; Liu, Yu-Lin; Ward, Carl; Wang, Tong; Zhang, Gong; Wang, Dongye; Yang, Jianhua; Chen, Yiwen; Zhang, Chaolin; Jauch, Ralf; Yang, Yun-Gui; Wang, Yangming; Qin, Baoming; Anko, Minna-Liisa; Hutchins, Andrew P.; Sun, Hao; Wang, Huating; Fu, Xiang-Dong; Zhang, Biliang; Esteban, Miguel A.
- Issue Date
- Mar-2018
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- NATURE METHODS, v.15, no.3, pp.213 - +
- Journal Title
- NATURE METHODS
- Volume
- 15
- Number
- 3
- Start Page
- 213
- End Page
- +
- ISSN
- 1548-7091
- Abstract
- We combine the labeling of newly transcribed RNAs with 5-ethynyluridine with the characterization of bound proteins. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs, including nascent RNAs and traditionally neglected nonpolyadenylated RNAs. RICK has identified mitotic regulators amongst other novel RNA-binding proteins with preferential affinity for nonpolyadenylated RNAs, revealed a link between metabolic enzymes/factors and nascent RNAs, and expanded the known RNA-bound proteome of mouse embryonic stem cells. RIC K will facilitate an in-depth interrogation of the total RNA-bound proteome in different cells and systems.
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Related Researcher
- Lim, Dohwan
- College of Natural Sciences (Department of Bioinformatics & Life Science)