Detailed Information
Cited 13 time in 
Cited 0 time in 
Cited 0 time in
Metadata Downloads
Microinjection free delivery of miRNA inhibitor into zygotes
- Authors
- Joo, Jin Young; Lee, Jonghwan; Ko, Hae Young; Lee, Yong Seung; Lim, Do-Hwan; Kim, Eun-Young; Cho, Sujeong; Hong, Ki-Sung; Ko, Jung Jae; Lee, Suman; Lee, Young Sik; Choi, Youngsok; Lee, Kyung-Ah; Kim, Soonhag
- Issue Date
- Jun-2014
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- SCIENTIFIC REPORTS, v.4
- Journal Title
- SCIENTIFIC REPORTS
- Volume
- 4
- ISSN
- 2045-2322
- Abstract
- The development of gene delivery systems into embryos is challenging due to technical difficulties, delivery efficiency and toxicity. Here, we developed an organic compound (VisuFect)-mediated gene delivery system for zygotes. The VisuFect, which is hydrophilic and Cy5.5-labeled, was conjugated with poly(A) oligo (VFA). The VFA into CHO cells showed clathrin-mediated internalization and no toxicity. The VFA successfully penetrated through the zona pellucida of fertilized eggs of various species including pigs, zebrafish, drosophilas and mice. The experiment with VisuFect-mediated delivery of the miR34c inhibitor showed similar results with direct microinjection of the miR34c inhibitor by suppressing the development of zygotes up to the blastocyst stage. Noticeable features of the VisuFect will provide great benefits for further studies on gene function in sperms and embryos.
- Files in This Item
- There are no files associated with this item.
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Related Researcher
- Lim, Dohwan
- College of Natural Sciences (Department of Bioinformatics & Life Science)