Application of Random Mutagenesis and Synthetic FadR Promoter for de novo Production of ω-Hydroxy Fatty Acid in Yarrowia lipolytica
DC Field | Value | Language |
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dc.contributor.author | Park, B.G. | - |
dc.contributor.author | Kim, J. | - |
dc.contributor.author | Kim, E.-J. | - |
dc.contributor.author | Kim, Y. | - |
dc.contributor.author | Kim, J. | - |
dc.contributor.author | Kim, J.Y. | - |
dc.contributor.author | Kim, B.-G. | - |
dc.date.available | 2021-03-19T06:40:18Z | - |
dc.date.created | 2021-03-19 | - |
dc.date.issued | 2021-02-22 | - |
dc.identifier.issn | 2296-4185 | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/40714 | - |
dc.description.abstract | As a means to develop oleaginous biorefinery, Yarrowia lipolytica was utilized to produce ω-hydroxy palmitic acid from glucose using evolutionary metabolic engineering and synthetic FadR promoters for cytochrome P450 (CYP) expression. First, a base strain was constructed to produce free fatty acids (FFAs) from glucose using metabolic engineering strategies. Subsequently, through ethyl methanesulfonate (EMS)-induced random mutagenesis and fluorescence-activated cell sorting (FACS) screening, improved FFA overproducers were screened. Additionally, synthetic promoters containing bacterial FadR binding sequences for CYP expression were designed to respond to the surge of the concentration of FFAs to activate the ω-hydroxylating pathway, resulting in increased transcriptional activity by 14 times from the third day of culture compared to the first day. Then, endogenous alk5 was screened and expressed using the synthetic FadR promoter in the developed strain for the production of ω-hydroxy palmitic acid. By implementing the synthetic FadR promoter, cell growth and production phases could be efficiently decoupled. Finally, in batch fermentation, we demonstrated de novo production of 160 mg/L of ω-hydroxy palmitic acid using FmeN3-TR1-alk5 in nitrogen-limited media. This study presents an excellent example of the production of ω-hydroxy fatty acids using synthetic promoters with bacterial transcriptional regulator (i.e., FadR) binding sequences in oleaginous yeasts. © Copyright © 2021 Park, Kim, Kim, Kim, Kim, Kim and Kim. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Frontiers Media S.A. | - |
dc.relation.isPartOf | Frontiers in Bioengineering and Biotechnology | - |
dc.title | Application of Random Mutagenesis and Synthetic FadR Promoter for de novo Production of ω-Hydroxy Fatty Acid in Yarrowia lipolytica | - |
dc.type | Article | - |
dc.identifier.doi | 10.3389/fbioe.2021.624838 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Frontiers in Bioengineering and Biotechnology, v.9 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000626029500001 | - |
dc.identifier.scopusid | 2-s2.0-85102307491 | - |
dc.citation.title | Frontiers in Bioengineering and Biotechnology | - |
dc.citation.volume | 9 | - |
dc.contributor.affiliatedAuthor | Kim, J. | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordAuthor | evolutionary metabolic engineering | - |
dc.subject.keywordAuthor | FadR | - |
dc.subject.keywordAuthor | synthetic promoter | - |
dc.subject.keywordAuthor | Yarrowia lipolytica | - |
dc.subject.keywordAuthor | ω-hydroxy fatty acid | - |
dc.subject.keywordPlus | Cell proliferation | - |
dc.subject.keywordPlus | Flow cytometry | - |
dc.subject.keywordPlus | Glucose | - |
dc.subject.keywordPlus | Metabolic engineering | - |
dc.subject.keywordPlus | Metabolism | - |
dc.subject.keywordPlus | Mutagenesis | - |
dc.subject.keywordPlus | Saturated fatty acids | - |
dc.subject.keywordPlus | Transcription | - |
dc.subject.keywordPlus | De novo production | - |
dc.subject.keywordPlus | Ethyl methanesulfonate | - |
dc.subject.keywordPlus | Fluorescence activated cell sorting | - |
dc.subject.keywordPlus | Hydroxy fatty acids | - |
dc.subject.keywordPlus | Random mutagenesis | - |
dc.subject.keywordPlus | Transcriptional activity | - |
dc.subject.keywordPlus | Transcriptional regulator | - |
dc.subject.keywordPlus | Yarrowia lipolytica | - |
dc.subject.keywordPlus | Palmitic acid | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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