Expression of soluble recombinant human matrix metalloproteinase 9 and generation of its monoclonal antibody
DC Field | Value | Language |
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dc.contributor.author | Jeong, Hee-Jin | - |
dc.contributor.author | Kim, Eun-Jung | - |
dc.contributor.author | Kim, Joo-Kyung | - |
dc.contributor.author | Kim, Yun-Gon | - |
dc.contributor.author | Lee, Chang-Soo | - |
dc.contributor.author | Ko, Byoung Joon | - |
dc.contributor.author | Kim, Byung-Gee | - |
dc.date.accessioned | 2021-11-08T06:40:04Z | - |
dc.date.available | 2021-11-08T06:40:04Z | - |
dc.date.created | 2021-11-08 | - |
dc.date.issued | 2021-11 | - |
dc.identifier.issn | 1046-5928 | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/41402 | - |
dc.description.abstract | We have successfully produced a recombinant human matrix metalloproteinase 9 (hMMP9) antigen with high yield and purity and used it to generate a hybridoma cell-culture-based monoclonal anti-hMMP9 antibody. We selected the most effective antibody for binding antigens and successfully identified its nucleotide sequence. The entire antigen and antibody developmental procedures described herein can be a practical approach for producing large amounts of monoclonal antibodies against hMMP9 and other antigens of interest. Additionally, the nucleotide sequence information of the anti-hMMP9 monoclonal antibody revealed herein will be useful for the generation of recombinant antibodies or antibody fragments against hMMP9. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | PROTEIN EXPRESSION AND PURIFICATION | - |
dc.title | Expression of soluble recombinant human matrix metalloproteinase 9 and generation of its monoclonal antibody | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.pep.2021.105931 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | PROTEIN EXPRESSION AND PURIFICATION, v.187 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000688289900003 | - |
dc.identifier.scopusid | 2-s2.0-85109535288 | - |
dc.citation.title | PROTEIN EXPRESSION AND PURIFICATION | - |
dc.citation.volume | 187 | - |
dc.contributor.affiliatedAuthor | Kim, Yun-Gon | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordAuthor | Matrix metalloproteinase 9 | - |
dc.subject.keywordAuthor | Recombinant protein | - |
dc.subject.keywordAuthor | Monoclonal antibody | - |
dc.subject.keywordAuthor | Hybridoma technology | - |
dc.subject.keywordPlus | C-TERMINAL DOMAIN | - |
dc.subject.keywordPlus | MATRIX METALLOPROTEINASES | - |
dc.subject.keywordPlus | SUBSTRATE-SPECIFICITY | - |
dc.subject.keywordPlus | BINDING DOMAIN | - |
dc.subject.keywordPlus | COLLAGENASE | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | INHIBITOR | - |
dc.subject.keywordPlus | MMP-9 | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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