Simultaneous determination of hydrochlorothiazide, amlodipine, and telmisartan with spectrophotometric and HPLC green chemistry applications
- Authors
- Tiris, Gizem; Mehmandoust, Mohammad; Lotfy, Hayam M.; Erk, Nevin; Joo, Sang-Woo; Dragoi, Elena-Niculina; Vasseghian, Yasser
- Issue Date
- Sep-2022
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Antihypertensive drugs; Amlodipine; Telmisartan; Hydrochlorothiazide; HPLC; Spectrophotometry
- Citation
- CHEMOSPHERE, v.303
- Journal Title
- CHEMOSPHERE
- Volume
- 303
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/42565
- DOI
- 10.1016/j.chemosphere.2022.135074
- ISSN
- 0045-6535
- Abstract
- For the quantifiable amounts of Telmisartan (TLM) and Hydrochlorothiazide (HYD) in the presence of Amlodipine (AML) in a ternary mixture of synthetic laboratory mixture, a novel, sensitive, quick, and practical reversed-phase high-performance liquid chromatography (RP-HPLC) method was given. In order to separate, a Waters Spherisorb ODS-2 C18 column was used. For HYD, TLM, and AML, these techniques were viable over linearity ranges of 4-12 mu g/mL, 4-25 mu g/mL, and 5-40 mu g/mL, respectively. The mobile phase system was acetonitrile:methanol: phosphate buffer at pH 2.5 (65:5:30 v/v/v), and the flow rate was 1.5 mL/min. Novel spectrophotometric methods were applied for active substances to determine simultaneously. The first method is absorptivity centering using factorized spectrum, and the second method is dual amplitude difference coupled
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