Raman Thermometry Nanopipettes in Cancer Photothermal Therapy
- Authors
- Dinh Nghi Ngo; Vuong Thi Thanh Xuan Ho; Kim, Gun; Song, Min Seok; Kim, Mi Ri; Choo, Jaebum; Joo, Sang-Woo; Lee, So Yeong
- Issue Date
- May-2022
- Publisher
- AMER CHEMICAL SOC
- Citation
- ANALYTICAL CHEMISTRY, v.94, no.17, pp.6463 - 6472
- Journal Title
- ANALYTICAL CHEMISTRY
- Volume
- 94
- Number
- 17
- Start Page
- 6463
- End Page
- 6472
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/42626
- DOI
- 10.1021/acs.analchem.1c04452
- ISSN
- 0003-2700
- Abstract
- Raman thermometry based on surface-enhanced Raman scattering has been developed using nanopipettes in cancer cell photothermal therapy (PTT). Gold nanorods (AuNRs) are robustly epoxied on glass pipettes with a high surface coverage of similar to 95% and less than 10 nm-wide nanogaps for intracellular thermometry and photothermal cancer therapy. The temperature changes could be estimated from the N equivalent to C band shifts of 4-fluorophenyl isocyanide (FPNC)-adsorbed AuNRs on the Raman thermometry nanopipette (RTN) surfaces. An intracellular temperature change of similar to 2.7 degrees C produced by altering the [Ca2+] in A431 cells was detected using the RTN in vitro, as checked from fura-2 acetoxymethyl ester (fura-2 AM) fluorescence images. For in vivo experiments, local temperature rises of similar to 19.2 degrees C were observed in the mouse skin, whereas infrared camera images could not tract due to spatial resolution. In addition, a tumor growth suppression was observed in the PTT processes after an administration of the three AuNR-coated nanopipettes combined with a 671 nm laser irradiation for 5 min in 30 days. These results demonstrate not only the localized temperature sensing ability of FPNC-tagged AuNR nanopipettes in cell biology but also anti-cancer effects in photothermal cancer therapy.
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