DNA-based artificial dendritic cells for in situ cytotoxic T cell stimulation and immunotherapy
DC Field | Value | Language |
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dc.contributor.author | Quoc-Viet Le | - |
dc.contributor.author | Lee, Jaiwoo | - |
dc.contributor.author | Byun, Junho | - |
dc.contributor.author | Shim, Gayong | - |
dc.contributor.author | Oh, Yu-Kyoung | - |
dc.date.accessioned | 2022-12-28T09:40:05Z | - |
dc.date.available | 2022-12-28T09:40:05Z | - |
dc.date.created | 2022-12-28 | - |
dc.date.issued | 2022-09 | - |
dc.identifier.issn | 2452-199X | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/42959 | - |
dc.description.abstract | In immunotherapy, ex vivo stimulation of T cells requires significant resources and effort. Here, we report artificial dendritic cell-mimicking DNA microflowers (DM) for programming T cell stimulation in situ. To mimic dendritic cells, DNA-based artificial dendritic microflowers were constructed, surface-coated with polydopamine, and further modified with anti-CD3 and anti-CD28 antibodies to yield antibody-modified DM (DM-A). The porous structure of DM-A allowed entrapment of the T cell-stimulating cytokine, ineterleukin-2, yielding interleukin-2-loaded DM-A (DM-AI). For comparison, polystyrene microparticles coated with polydopamine and modified with anti-CD3 and anti-CD28 antibodies (PS-A) were used. Compared to PS-A, DM-AI showed significantly greater contact with T cell surfaces. DM-AI provided the highest ex vivo expansion of cytotoxic T cells. Local injection of DM-AI to tumor tissues induced the recruitment of T cells and expansion of cytotoxic T cells in tumor microenvironments. Unlike the other groups, model animals injected with DM-AI did not exhibit growth of primary tumors. Treatment of mice with DM-AI also protected against growth of a rechallenged distant tumor, and thus prevented tumor recurrence in this model. DM-AI has great potential for programmed stimulation of CD8(+) T cells. This concept could be broadly extended for the programming of specific T cell stimulation profiles. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KEAI PUBLISHING LTD | - |
dc.relation.isPartOf | BIOACTIVE MATERIALS | - |
dc.title | DNA-based artificial dendritic cells for in situ cytotoxic T cell stimulation and immunotherapy | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bioactmat.2021.12.001 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOACTIVE MATERIALS, v.15, pp.160 - 172 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000788665800003 | - |
dc.identifier.scopusid | 2-s2.0-85121813363 | - |
dc.citation.endPage | 172 | - |
dc.citation.startPage | 160 | - |
dc.citation.title | BIOACTIVE MATERIALS | - |
dc.citation.volume | 15 | - |
dc.contributor.affiliatedAuthor | Shim, Gayong | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S2452199X21005533?via%3Dihub | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.subject.keywordAuthor | Artificial dendritic cell | - |
dc.subject.keywordAuthor | DNA microflower | - |
dc.subject.keywordAuthor | In situ T cell stimulation | - |
dc.subject.keywordAuthor | Programmed T cell expansion | - |
dc.subject.keywordAuthor | Immunotherapy | - |
dc.subject.keywordPlus | ANTIGEN-PRESENTING CELLS | - |
dc.subject.keywordPlus | EX-VIVO EXPANSION | - |
dc.subject.keywordPlus | DESIGN | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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