A MALDI-MS-based quantitative glycoprofiling method on a 96-well plate platform

Abstract

Here, we developed a high-throughput MALDI-MS based quantitative targeted glycomics (HT MALDI-QTaG) method for analyzing total N-glycans. Although the chemical derivatization processes (i.e., neutralization of sialic acid and incorporation of a positively-charged moiety) were performed in a 96-well with built-in 10 kDa MWCO membrane filters, the quantitative linearity was still quite good (R-2 > 0.99) between varying amounts of the target glycoprotein and the MALDI peak intensities. In addition, we validated the relative quantitative reproducibilities in different well positions in a 96-well plate. As a proof-of-concept, the proposed HT MALDI-QTaG method was successfully used to analyze bovine fetuin, human serum and Enbrel (R). (C) 2016 Published by Elsevier B.V. on behalf of The Korean Society of Industrial and Engineering Chemistry.