국내 자생 고로쇠 (Acer mono Max.) 추출물의 항산화 활성

Abstract

Maple tree is a useful medical plant for obtaining bioactive materials such as pharmaceutics, cosmetics, food additive, etc., and there are 16 species of native maple trees in Korea. In this study, we evaluated the antioxidant activity of sap and crude extracts of Acer mono Max, a representative maple species. The crude extracts were obtained by solvent extraction (water, ethanol, and ethyl acetate) from its branches (bark and xylem). The phenolic contents and radical scavenging capacities of the extracts and the sap were evaluated in terms of half maximal effective concentration (EC50) and kinetics by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The ethanol extracts showed the highest extraction yield, phenolic contents and antioxidant activity, and bark extracts showed better antioxidant activity than xylem extracts. The antioxidant activity of the sap was very low, but the EC50 of ethanol and ethyl acetate extracts ranged from 68 to 79 μg/mL, similar to that (60 μg/mL) of the control, butylated hydroxytoluene (BHT). The DPPH radical scavenging rate (220~760 μM/ min) and the second-order reaction rate constant (6.48~7.04 L/g·min) of these extracts were better than those of BHT (55~ 370 μM/min and 3.60 L/g·min). These results suggest that A. mono Max. is one of the useful bioresources for obtaining antioxidant biologically active substances, and it is possible to obtain physiologically active substances from by-product of its pruning while minimizing the effect on the growth of the tree.