D-Galactose as an autoinducer 2 inhibitor to control the biofilm formation of periodontopathogens
- Authors
- Ryu, Eun-Ju; Sim, Jaehyun; Sim, Jun; Lee, Julian; Choi, Bong-Kyu
- Issue Date
- Sep-2016
- Publisher
- MICROBIOLOGICAL SOCIETY KOREA
- Keywords
- quorum sensing; periodontopathogens; biofilm; AI-2 inhibitor; D-galactose
- Citation
- JOURNAL OF MICROBIOLOGY, v.54, no.9, pp.632 - 637
- Journal Title
- JOURNAL OF MICROBIOLOGY
- Volume
- 54
- Number
- 9
- Start Page
- 632
- End Page
- 637
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/7514
- DOI
- 10.1007/s12275-016-6345-8
- ISSN
- 1225-8873
- Abstract
- Autoinducer 2 (AI-2) is a quorum sensing molecule to which bacteria respond to regulate various phenotypes, including virulence and biofilm formation. AI-2 plays an important role in the formation of a subgingival biofilm composed mostly of Gram-negative anaerobes, by which periodontitis is initiated. The aim of this study was to evaluate D-galactose as an inhibitor of AI-2 activity and thus of the biofilm formation of periodontopathogens. In a search for an AI-2 receptor of Fusobacterium nucleatum, D-galactose binding protein (Gbp, Gene ID FN1165) showed high sequence similarity with the ribose binding protein (RbsB), a known AI-2 receptor of Aggregatibacter actinomycetemcomitans. D-Galactose was evaluated for its inhibitory effect on the AI-2 activity of Vibrio harveyi BB152 and F. nucleatum, the major coaggregation bridge organism, which connects early colonizing commensals and late pathogenic colonizers in dental biofilms. The inhibitory effect of D-galactose on the biofilm formation of periodontopathogens was assessed by crystal violet staining and confocal laser scanning microscopy in the absence or presence of AI-2 and secreted molecules of F. nucleatum. D-Galactose significantly inhibited the AI-2 activity of V. harveyi and F. nucleatum. In addition, D-galactose markedly inhibited the biofilm formation of F. nucleatum, Porphyromonas gingivalis, and Tannerella forsythia induced by the AI-2 of F. nucleatum without affecting bacterial growth. Our results demonstrate that the Gbp may function as an AI-2 receptor and that galactose may be used for prevention of the biofilm formation of periodontopathogens by targeting AI-2 activity.
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Collections - College of Natural Sciences > School of Systems and Biomedical Science > 1. Journal Articles
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