A MALDI-MS-based quantitative analytical method for endogenous estrone in human breast cancer cells
- Authors
- Kim, Kyoung-Jin; Kim, Hee-Jin; Park, Han-Gyu; Hwang, Cheol-Hwan; Sung, Changmin; Jang, Kyoung-Soon; Park, Sung-Hee; Kim, Byung-Gee; Lee, Yoo-Kyung; Yang, Yung-Hun; Jeong, Jae Hyun; Kim, Yun-Gon
- Issue Date
- 19-Apr-2016
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- SCIENTIFIC REPORTS, v.6
- Journal Title
- SCIENTIFIC REPORTS
- Volume
- 6
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/7634
- DOI
- 10.1038/srep24489
- ISSN
- 2045-2322
- Abstract
- The level of endogenous estrone, one of the three major naturally occurring estrogens, has a significant correlation with the incidence of post-menopausal breast cancer. However, it is challenging to quantitatively monitor it owing to its low abundance. Here, we develop a robust and highly sensitive mass-assisted laser desorption/ionization mass spectrometry (MALDI-MS)-based quantitative platform to identify the absolute quantities of endogenous estrones in a variety of clinical specimens. The one-step modification of endogenous estrone provided good linearity (R-2 > 0.99) and significantly increased the sensitivity of the platform (limit of quantitation: 11 fmol). In addition, we could identify the absolute amount of endogenous estrones in cells of the breast cancer cell line MCF-7 (34 fmol/10(6) cells) by using a deuterated estrone as an internal standard. Finally, by applying the MALDI-MS-based quantitative method to endogenous estrones, we successfully monitored changes in the metabolic expression level of estrones (17.7 fmol/10(6) letrozole-treated cells) in MCF-7 cells resulting from treatment with an aromatase inhibitor. Taken together, these results suggest that this MALDI-MS-based quantitative approach may be a general method for the targeted metabolomics of ketone-containing metabolites, which can reflect clinical conditions and pathogenic mechanisms.
- Files in This Item
-
Go to Link
- Appears in
Collections - College of Engineering > Department of Chemical Engineering > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.