Rhodium Complex and Enzyme Couple Mediated Electrochemical Detection of Adenosine
- Authors
- Han, Dawoon; Kim, Hyeong-Mook; Chand, Rohit; Kim, Gyumin; Shin, Ik-Soo; Kim, Yong-Sang
- Issue Date
- Oct-2015
- Publisher
- HUMANA PRESS INC
- Keywords
- Adenosine; Enzymatic reaction; Electrochemical; Electron transfer mediator; Nafion
- Citation
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.177, no.4, pp.812 - 820
- Journal Title
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
- Volume
- 177
- Number
- 4
- Start Page
- 812
- End Page
- 820
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/8630
- DOI
- 10.1007/s12010-015-1779-8
- ISSN
- 0273-2289
- Abstract
- Adenosine is one of the nucleoside which plays an important role in signal transduction and neuromodulation. This work proposes a simple electrochemical assay, comprising two enzymes and rhodium complex based electron transfer mediator, for the detection of adenosine. Sequential reaction of adenosine deaminase and l-glutamic dehydrogenase and the supporting cycle between beta-NADH and mediator enable quantitative analysis of adenosine. Role of electron transfer mediator is the conveyance of proton from electrode to beta-NAD(+) for regeneration of beta-NADH. The electrochemical characteristics of electron transfer mediator were also studied. Real-time adenosine detection was carried out using this multiple enzyme based chronoamperometric assay. The analysis results show a low limit of detection (140 mu M) and good correspondence between current signal and the adenosine concentration (R-2 = 0.997).
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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