An On-Line Platform for the Analysis of Permethylated Oligosaccharides Using Capillary Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry
DC Field | Value | Language |
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dc.contributor.author | Jang, Kyoung-Soon | - |
dc.contributor.author | Park, Hae-Min | - |
dc.contributor.author | Kim, Yun-Gon | - |
dc.contributor.author | Kim, Byung-Gee | - |
dc.contributor.author | Kim, June-Hyung | - |
dc.date.available | 2018-05-09T07:36:11Z | - |
dc.date.created | 2018-04-17 | - |
dc.date.issued | 2015-05 | - |
dc.identifier.issn | 1533-4880 | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/8738 | - |
dc.description.abstract | Permethylation is one of the most practical modifications for mass spectrometric analysis of carbohydrates. In this study, we showed a facile platform for the on-line permethylation and analysis of acidic N-glycans using capillary liquid chromatography-electrospray ionization-tandem mass spectrometry to elucidate the N-glycan structures derived from glycoprotein samples. Two capillaries, individually packed with NaOH and C-18 powder, were coupled to electrospray ion trap mass spectrometer. Prior to mass spectrometry, oligosaccharides were permethylated in NaOH-packed capillary by methyl iodide, and the permethylated glycans were consequently extracted from residual reagents, such as NaOH and methyl iodide, in C-18-capillary, instead of using conventional chloroform extraction. After permethylation and purification, the permethylated glycans were released from C-18 capillary by acetonitrile, and sprayed into mass spectrometer. Using this on-line strategy, a picomolar range of sialylated N-glycans from bovine fetuin were analyzed by tandem mass spectrometry. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | - |
dc.relation.isPartOf | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.title | An On-Line Platform for the Analysis of Permethylated Oligosaccharides Using Capillary Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry | - |
dc.type | Article | - |
dc.identifier.doi | 10.1166/jnn.2015.9534 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.15, no.5, pp.3962 - 3966 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000347435200086 | - |
dc.identifier.scopusid | 2-s2.0-84920746951 | - |
dc.citation.endPage | 3966 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 3962 | - |
dc.citation.title | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.citation.volume | 15 | - |
dc.contributor.affiliatedAuthor | Kim, Yun-Gon | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordAuthor | Oligosaccharide | - |
dc.subject.keywordAuthor | On-Line | - |
dc.subject.keywordAuthor | Permethylation | - |
dc.subject.keywordAuthor | Capillary Electrospray Ionization-Tandem Mass Spectrometry | - |
dc.subject.keywordAuthor | Sodium Hydroxide | - |
dc.subject.keywordPlus | COLLISION-INDUCED FRAGMENTATION | - |
dc.subject.keywordPlus | SOLID-PHASE PERMETHYLATION | - |
dc.subject.keywordPlus | FAST-ATOM-BOMBARDMENT | - |
dc.subject.keywordPlus | STRUCTURAL-ANALYSIS | - |
dc.subject.keywordPlus | INDUCED DISSOCIATION | - |
dc.subject.keywordPlus | N-GLYCANS | - |
dc.subject.keywordPlus | MATRIX | - |
dc.subject.keywordPlus | GLYCOMICS | - |
dc.subject.keywordPlus | MS | - |
dc.subject.keywordPlus | NOMENCLATURE | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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