Stable Isotopic Labeling-Based Quantitative Targeted Glycomics (i-QTaG)
DC Field | Value | Language |
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dc.contributor.author | Kim, Kyoung-Jin | - |
dc.contributor.author | Kim, Yoon-Woo | - |
dc.contributor.author | Kim, Yun-Gon | - |
dc.contributor.author | Park, Hae-Min | - |
dc.contributor.author | Jin, Jang Mi | - |
dc.contributor.author | Kim, Young Hwan | - |
dc.contributor.author | Yang, Yung-Hun | - |
dc.contributor.author | Lee, Jun Kyu | - |
dc.contributor.author | Chung, Junho | - |
dc.contributor.author | Lee, Sun-Gu | - |
dc.contributor.author | Saghatelian, Alan | - |
dc.date.available | 2018-05-09T07:36:29Z | - |
dc.date.created | 2018-04-17 | - |
dc.date.issued | 2015-05 | - |
dc.identifier.issn | 8756-7938 | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/8746 | - |
dc.description.abstract | Mass spectrometry (MS) analysis combined with stable isotopic labeling is a promising method for the relative quantification of aberrant glycosylation in diseases and disorders. We developed a stable isotopic labeling-based quantitative targeted glycomics (i-QTaG) technique for the comparative and quantitative analysis of total N-glycans using matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS). We established the analytical procedure with the chemical derivatizations (i. e., sialic acid neutralization and stable isotopic labeling) of N-glycans using a model glycoprotein (bovine fetuin). Moreover, the i-QTaG using MALDI-TOF MS was evaluated with various molar ratios (1: 1, 1: 2, 1: 5) of (13) C-6/ (12) C-6-2-aminobenzoic acid-labeled glycans from normal human serum. Finally, this method was applied to direct comparison of the total N-glycan profiles between normal human sera (n=8) and prostate cancer patient sera (n=17). The intensities of the N-glycan peaks from i-QTaG method showed a good linearity (R-2 > 0.99) with the amount of the bovine fetuin glycoproteins. The ratios of relative intensity between the isotopically 2-AA labeled N-glycans were close to the theoretical molar ratios (1: 1, 1: 2, 1: 5). We also demonstrated that the up-regulation of the Lewis antigen (similar to 82%) in sera from prostate cancer patients. In this proof-of-concept study, we demonstrated that the i-QTaG method, which enables to achieve a reliable comparative quantitation of total N-glycans via MALDI-TOF MS analysis, has the potential to diagnose and monitor alterations in glycosylation associated with disease states or biotherapeutics. (C) 2015 American Institute of Chemical Engineers | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.relation.isPartOf | BIOTECHNOLOGY PROGRESS | - |
dc.subject | PROSTATE-SPECIFIC ANTIGEN | - |
dc.subject | FLIGHT MASS-SPECTROMETRY | - |
dc.subject | SIALYLATED N-GLYCANS | - |
dc.subject | SIALIC ACIDS | - |
dc.subject | MALDI-MS | - |
dc.subject | LINKED OLIGOSACCHARIDES | - |
dc.subject | MATRIX | - |
dc.subject | GLYCOSYLATION | - |
dc.subject | CANCER | - |
dc.subject | QUANTIFICATION | - |
dc.title | Stable Isotopic Labeling-Based Quantitative Targeted Glycomics (i-QTaG) | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/btpr.2078 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY PROGRESS, v.31, no.3, pp.840 - 848 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000356670700028 | - |
dc.identifier.scopusid | 2-s2.0-84931576539 | - |
dc.citation.endPage | 848 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 840 | - |
dc.citation.title | BIOTECHNOLOGY PROGRESS | - |
dc.citation.volume | 31 | - |
dc.contributor.affiliatedAuthor | Kim, Yun-Gon | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | MALDI-MS | - |
dc.subject.keywordAuthor | N-glycan | - |
dc.subject.keywordAuthor | comparative quantitation | - |
dc.subject.keywordAuthor | stable isotopic labeling | - |
dc.subject.keywordAuthor | sialic acid neutralization | - |
dc.subject.keywordPlus | PROSTATE-SPECIFIC ANTIGEN | - |
dc.subject.keywordPlus | FLIGHT MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | SIALYLATED N-GLYCANS | - |
dc.subject.keywordPlus | SIALIC ACIDS | - |
dc.subject.keywordPlus | MALDI-MS | - |
dc.subject.keywordPlus | LINKED OLIGOSACCHARIDES | - |
dc.subject.keywordPlus | MATRIX | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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